Ouellette M, Gerbaud G, Lambert T, Courvalin P
Unité des Agents Antibactériens, Centre National de la Recherche Scientifique, Institut Pasteur, France.
Antimicrob Agents Chemother. 1987 Jul;31(7):1021-6. doi: 10.1128/AAC.31.7.1021.
A Campylobacter-like organism, BM2196, resistant to kanamycin and streptomycin-spectinomycin was isolated from the feces of a patient with acute enteritis. The kanamycin and streptomycin-spectinomycin resistances were not transferable to Camplylobacter sp. or to Escherichia coli, and no plasmid DNA was detected in this strain. The resistance genes were therefore tentatively assigned to a chromosomal locality. Analysis by the phosphocellulose paper-binding assay of extracts from BM2196 indicated that resistance to kanamycin and structurally related antibiotics was due to the synthesis of 3'-aminoglycoside phosphotransferase type I [APH(3')-I], an enzyme specific for gram-negative bacteria, and that resistance to streptomycin-spectinomycin was secondary to the presence of a 3",9-aminoglycoside adenylyltransferase. Homology between BM2196 and an APH(3')-I probe was detected by DNA-DNA hybridization. A 2.2-kilobase BM2196 DNA fragment conferring resistance to kanamycin was cloned in E. coli and was sequenced partially. The resistance gene appeared nearly identical to that of Tn903 from E. coli and was adjacent to IS15-delta, an insertion sequence widespread in gram-negative bacteria, thus indicating that Campylobacter species can act as a recipient for genes originating in members of the family Enterobacteriaceae.
从一名急性肠炎患者的粪便中分离出一种类似弯曲杆菌的生物体BM2196，它对卡那霉素和链霉素-壮观霉素具有抗性。卡那霉素和链霉素-壮观霉素抗性不能转移至弯曲杆菌属或大肠杆菌，并且在该菌株中未检测到质粒DNA。因此，抗性基因暂时被定位在染色体上。通过对BM2196提取物进行磷酸纤维素纸结合试验分析表明，对卡那霉素和结构相关抗生素的抗性是由于I型3'-氨基糖苷磷酸转移酶[APH(3')-I]的合成，该酶是革兰氏阴性菌特有的一种酶，而对链霉素-壮观霉素的抗性是由于存在3",9-氨基糖苷腺苷酸转移酶。通过DNA-DNA杂交检测到BM2196与APH(3')-I探针之间存在同源性。将一个赋予卡那霉素抗性的2.2千碱基BM2196 DNA片段克隆到大肠杆菌中并进行了部分测序。该抗性基因似乎与来自大肠杆菌的Tn903的抗性基因几乎相同，并且与IS15-δ相邻，IS15-δ是一种在革兰氏阴性菌中广泛存在的插入序列，这表明弯曲杆菌属可作为源自肠杆菌科成员的基因的受体。
