Urbic Tomaz, Najem Sara, Dias Cristiano L
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Vecna pot 113, 1000, Slovenia.
National Center for Remote Sensing, National Council for Scientific Research (CNRS), Riad al Soloh, 1107 2260 Beirut, Lebanon.
Biophys Chem. 2017 Dec;231:155-160. doi: 10.1016/j.bpc.2017.03.001. Epub 2017 Mar 7.
In this manuscript we use a two-dimensional coarse-grained model to study how amyloid fibrils grow towards an equilibrium state where they coexist with proteins dissolved in a solution. Free-energies to dissociate proteins from fibrils are estimated from the residual concentration of dissolved proteins. Consistent with experiments, the concentration of proteins in solution affects the growth rate of fibrils but not their equilibrium state. Also, studies of the temperature dependence of the equilibrium state can be used to estimate thermodynamic quantities, e.g., heat capacity and entropy.
在本手稿中，我们使用二维粗粒化模型来研究淀粉样纤维如何生长至与溶解在溶液中的蛋白质共存的平衡状态。从溶解蛋白质的残余浓度估算将蛋白质从纤维上解离的自由能。与实验结果一致，溶液中蛋白质的浓度会影响纤维的生长速率，但不影响其平衡状态。此外，对平衡状态的温度依赖性研究可用于估算热力学量，例如热容和熵。
