Agroscope, Federal Department of Economic Affairs, Education and Research EAER, CH-3003 Bern, Switzerland.
Bioinformatics Core Facility, SIB Swiss Institute of Bioinformatics, University of Lausanne, CH-1015, Lausanne, Switzerland.
J Nutr Biochem. 2017 May;43:156-165. doi: 10.1016/j.jnutbio.2017.02.012. Epub 2017 Mar 4.
We have investigated the postprandial transcriptional response of blood cells to increasing caloric doses of a meal challenge to test whether the dynamic response of the human organism to the ingestion of food is dependent on metabolic health. The randomized crossover study included seven normal weight and seven obese men consuming three doses (500/1000/1500 kcal) of a high-fat meal. The blood cell transcriptome was measured before and 2, 4, and 6 h after meal ingestion (168 samples). We applied univariate and multivariate statistics to investigate differentially expressed genes in both study groups. We identified 624 probe sets that were up- or down-regulated after the caloric challenge in a dose-dependent manner. These transcripts were most responsive to the 1500 kcal challenge in the obese group and were associated with postprandial insulin and oxidative phosphorylation. Furthermore, the data revealed a separation of the obese group into individuals whose response was close to the normal weight group and individuals with a transcriptional response indicative of a loss of metabolic flexibility. The molecular signature provided by the postprandial transcriptomic response of blood cells to increasing caloric doses of a high-fat meal challenge may represent a sensitive way to evaluate the qualitative impact of food on human health.
我们研究了餐后血细胞对增加热量餐挑战的转录反应，以测试人体对食物摄入的动态反应是否依赖于代谢健康。这项随机交叉研究包括 7 名正常体重和 7 名肥胖男性，他们分别摄入三种剂量（500/1000/1500 卡路里）的高脂肪餐。在餐前和餐后 2、4 和 6 小时测量了血细胞转录组（共 168 个样本）。我们应用单变量和多变量统计学方法来研究两组中差异表达的基因。我们确定了 624 个探针集，它们在剂量依赖性的方式下被上调或下调。这些转录物对肥胖组的 1500 卡路里挑战最为敏感，与餐后胰岛素和氧化磷酸化有关。此外，数据显示肥胖组中的个体可以分为两类，一类的反应接近正常体重组，另一类的反应表明代谢灵活性丧失。餐后血细胞对增加热量餐挑战的转录反应提供的分子特征可能代表了一种评估食物对人体健康定性影响的敏感方法。
