Department of Respiratory Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong, 272019, China.
Department of Respiratory Medicine, Huai'an Second People's Hospital, Huai'an, Jiangsu, 223002, China.
Biomed Pharmacother. 2017 May;89:1227-1234. doi: 10.1016/j.biopha.2017.02.017. Epub 2017 Mar 17.
MicroRNA-448 (miR-448) has been showed to be low-expressed and function as tumor suppressor in most human cancers. However, there are limited reports on the clinical significance and biological function of miR-448 in lung squamous cell carcinoma. In this study, we observed that miR-448 expression was decreased in lung squamous cell carcinoma tissues and cell lines. Meanwhile, miR-448 expression associated with differentiated degree, T classification (tumor size), N classification (lymph node metastasis), M classification (distant metastasis), clinical stage and prognosis of lung squamous cell carcinoma patients. In survival analysis, low expression of miR-448 was a poor independent prognostic factor for lung squamous cell carcinoma patients. Moreover, gain-of-function and loss-of-function studies showed miR-448 acted as a tumor suppressor regulating lung squamous cell carcinoma cells growth and metastasis. Furthermore, DCLK1 has been identified as a potential target for miR-448 to regulate lung squamous cell carcinoma cells growth and metastasis. In conclusion, miR-448 low-expression was a poor prognostic factor for lung squamous cell carcinoma patients, and miR-448 served as a tumor suppressor in lung squamous cell carcinoma cells via targeting DCLK1.
微小 RNA-448（miR-448）在大多数人类癌症中表达水平较低，发挥着肿瘤抑制因子的作用。然而，关于 miR-448 在肺鳞癌中的临床意义和生物学功能的报道有限。在本研究中，我们观察到 miR-448 在肺鳞癌组织和细胞系中表达下调。同时，miR-448 的表达与肺鳞癌患者的分化程度、T 分类（肿瘤大小）、N 分类（淋巴结转移）、M 分类（远处转移）、临床分期和预后相关。生存分析表明，miR-448 低表达是肺鳞癌患者不良的独立预后因素。此外，功能获得和功能丧失研究表明，miR-448 作为肿瘤抑制因子调节肺鳞癌细胞的生长和转移。此外，已经鉴定出 DCLK1 是 miR-448 调节肺鳞癌细胞生长和转移的潜在靶标。总之，miR-448 低表达是肺鳞癌患者预后不良的因素，miR-448 通过靶向 DCLK1 在肺鳞癌细胞中发挥肿瘤抑制作用。
