de Oliveira Danilo B, Candiani Talitah M, Franco-Luiz Ana Paula M, Almeida Gabriel M F, Abrahão Jônatas S, Rios Maria, Coimbra Roney S, Kroon Erna G
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil; Faculdade de Medicina de Diamantina, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina 39100-000, Brazil.
Hospital Infantil João Paulo II, FHEMIG, Belo Horizonte 30130-110, Brazil.
J Neurol Sci. 2017 Apr 15;375:390-394. doi: 10.1016/j.jns.2017.02.025. Epub 2017 Feb 16.
Meningitis is a disease with a global distribution that constitutes a worldwide burden, with viruses as the primary etiologic agents. The range of viral meningitis severity depends mainly on age, immune status and etiological agent. The aim of this work was to investigate the suspected cases of viral meningitis using molecular techniques to confirm the viral infection. The diagnosed virus was correlated with clinical findings and cytochemical parameters in cerebrospinal liquid (CSF) of patients. CSF of 70 children with the presumptive diagnosis of viral meningitis was analyzed by real time PCR (qPCR). Viruses were identified by qPCR in 44 CSF samples (62.9%). Among them, 31 were identified as Enterovirus (ENTV) (70.4%), six as Human herpes virus 3 (HHV-3) (13.6%), five as Dengue virus (DENV) (11.7%), one as Human herpes virus 1-2 (2.3%) and one as Human herpes virus 5 (2.3%). Patients in the HHV-positive groups had increased percentage of polymorphonuclear neutrophils (PMN) (mean of 81%) while the groups of patients with DENV and ENTV had a mean of 30.9%. This study contributes to the knowledge of the epidemiological distribution of viral agents in CNS infections in children. In addition, it raises the relevance of DENV as an agent of CNS infection, and reinforces the importance for molecular in the cases of CNV infection.
脑膜炎是一种全球分布的疾病，构成了全球性负担，病毒是主要病因。病毒性脑膜炎的严重程度范围主要取决于年龄、免疫状态和病原体。这项工作的目的是使用分子技术调查疑似病毒性脑膜炎病例，以确认病毒感染。将诊断出的病毒与患者脑脊液（CSF）中的临床发现和细胞化学参数相关联。对70例初步诊断为病毒性脑膜炎的儿童的脑脊液进行实时PCR（qPCR）分析。通过qPCR在44份脑脊液样本（62.9%）中鉴定出病毒。其中，31例被鉴定为肠道病毒（ENTV）（70.4%），6例为人类疱疹病毒3型（HHV-3）（13.6%），5例为登革病毒（DENV）（11.7%），1例为人类疱疹病毒1-2型（2.3%），1例为人类疱疹病毒5型（2.3%）。HHV阳性组患者的多形核中性粒细胞（PMN）百分比增加（平均为81%），而DENV和ENTV组患者的平均百分比为30.9%。本研究有助于了解儿童中枢神经系统感染中病毒病原体的流行病学分布。此外，它提高了登革病毒作为中枢神经系统感染病原体的相关性，并强化了分子检测在中枢神经系统病毒感染病例中的重要性。
