Geteneh Alene, Kassa Tesfaye, Alemu Derbie, Kiros Mulugeta, Andualem Henok, Tenna Admasu, Tesfaye Abebech, Alemayehu Dawit Hailu, Mihret Adane, Howe Rawleigh, Mulu Andargachew, Mihret Wude
Department of Medical Laboratory Science, College of Health Science, Woldia University, Woldia, Ethiopia.
School of Medical Laboratory Science, Jimma University, Jimma, Ethiopia.
Infect Drug Resist. 2021 Mar 17;14:1083-1088. doi: 10.2147/IDR.S304521. eCollection 2021.
Viral meningitis is common in most resource-limited settings, posing a challenge for the management and prognosis of suspected patients. No study has been done on the detection of either viral or viral-bacterial co-infection among presumed pyogenic meningitis cases in Ethiopia. We, therefore, aimed to determine the distribution of cytomegalovirus (CMV) and human enteroviruses (HEVs) among patients with presumptive pyogenic meningitis at University hospitals in Ethiopia.
Viral nucleic acid was extracted from 86 repository CSF samples, which were collected from patients presumptively diagnosed with pyogenic meningitis between 2012 and 2013. PCR was done consecutively to investigate the possible viral etiologic agents of meningitis.
HEVs were detected in 11 (12.8%) of the analyzed samples while none of the 86 samples were tested positive for CMV. Viral-bacterial co-infections were found among 4/11 (36.4%) confirmed cases. The majority of the patients (10/11) with HEVs were younger aged ≤ 19 years old.
In this study, the magnitude of HEVs was shown to have a significant role in presumed pyogenic meningitis cases. Therefore, we recommend presumed pyogenic meningitis cases to be inspected for viral etiologies and improve meningeal symptoms interpretations.
在大多数资源有限的地区，病毒性脑膜炎很常见，这给疑似患者的管理和预后带来了挑战。埃塞俄比亚尚未对疑似化脓性脑膜炎病例中的病毒感染或病毒-细菌混合感染进行检测。因此，我们旨在确定埃塞俄比亚大学医院疑似化脓性脑膜炎患者中巨细胞病毒（CMV）和人肠道病毒（HEV）的分布情况。
从86份脑脊液储存样本中提取病毒核酸，这些样本采集自2012年至2013年间疑似诊断为化脓性脑膜炎的患者。连续进行聚合酶链反应（PCR）以调查脑膜炎可能的病毒病原体。
在11份（12.8%）分析样本中检测到HEV，而86份样本中没有一份CMV检测呈阳性。在4/11（36.4%）确诊病例中发现了病毒-细菌混合感染。大多数感染HEV的患者（10/11）年龄≤19岁。
在本研究中，HEV在疑似化脓性脑膜炎病例中显示出重要作用。因此，我们建议对疑似化脓性脑膜炎病例进行病毒病因检查，并改善脑膜症状的解读。
