莲（荷叶）提取物抑制小鼠气道平滑肌收缩。
Nelumbo nucifera leaves extracts inhibit mouse airway smooth muscle contraction.
作者信息
Yang Xiao, Xue Lu, Zhao Qingyang, Cai Congli, Liu Qing-Hua, Shen Jinhua
机构信息
Institute for Medical Biology and Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan, 430074, China.
Wuhan Youzhiyou Biopharmaceutical Co., Ltd., 666 Gaoxin Road, Biolake, Wuhan, 430075, China.
出版信息
BMC Complement Altern Med. 2017 Mar 20;17(1):159. doi: 10.1186/s12906-017-1674-7.
BACKGROUND
Alkaloids extracted from lotus leaves (AELL) can relax vascular smooth muscle. However, whether AELL has a similar relaxant role on airway smooth muscle (ASM) remains unknown. This study aimed to explore the relaxant property of AELL on ASM and the underlying mechanism.
METHODS
Alkaloids were extracted from dried lotus leaves using the high temperature rotary evaporation extraction method. The effects of AELL on mouse ASM tension were studied using force measuring and patch-clamp techniques.
RESULTS
It was found that AELL inhibited the high K or acetylcholine chloride (ACh)-induced precontraction of mouse tracheal rings by 64.8 ± 2.9%, or 48.8 ± 4.7%, respectively. The inhibition was statistically significant and performed in a dose-dependent manner. Furthermore, AELL-induced smooth muscle relaxation was partially mediated by blocking voltage-dependent Ca channels (VDCC) and non-selective cation channels (NSCC).
CONCLUSION
AELL, which plays a relaxant role in ASM, might be a new complementary treatment to treat abnormal contractions of the trachea and asthma.
背景
从荷叶中提取的生物碱（AELL）可使血管平滑肌松弛。然而，AELL对气道平滑肌（ASM）是否具有类似的松弛作用尚不清楚。本研究旨在探讨AELL对ASM的松弛特性及其潜在机制。
方法
采用高温旋转蒸发提取法从干荷叶中提取生物碱。运用测力和膜片钳技术研究AELL对小鼠ASM张力的影响。
结果
发现AELL分别使高钾或氯化乙酰胆碱（ACh）诱导的小鼠气管环预收缩抑制64.8±2.9%或48.8±4.7%。该抑制具有统计学意义且呈剂量依赖性。此外，AELL诱导的平滑肌松弛部分是通过阻断电压依赖性钙通道（VDCC）和非选择性阳离子通道（NSCC）介导的。
结论
在ASM中起松弛作用的AELL可能是治疗气管异常收缩和哮喘的一种新的辅助治疗方法。
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