Oliveira Maria Gabriela Xavier, Pressinotti Leandro Nogueira, Carvalho Giovane Spínola, Oliveira Mirela Caroline Vilela, Moreno Luisa Zanolli, Matajira Carlos Emilio Cabrera, Bergamo Alessandro Spínola, Aleixo Victor Manuel, Veiga Alexandre Caixeta, de Souza Corsino Elvis, Christ Ana Paula Guarnieri, Sato Maria Inês Zanolli, Moreno Andrea Micke, Knöbl Terezinha
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87-Cidade Universitária, São Paulo, SP, Brazil.
Universidade do Estado de Mato Grosso, Av. São João s/n-Cavalhada, Cáceres, MT, Spain.
Trop Anim Health Prod. 2017 Apr;49(4):777-782. doi: 10.1007/s11250-017-1262-3. Epub 2017 Mar 21.
The aim of this study was to perform the identification and molecular characterization of Arcobacter cryaerophilus and Arcobacter butzleri isolated from caiman (Caiman yacare), kept at a production farm, in Brazil. Forty fecal samples were analyzed. After isolation and identification, 21/40 strains of A. butzleri and 19/40 strains of A. cryaerophilus were subjected to PCR for potential virulence gene detection. The results of the PCR showed 38/40 strains positive for the cadF, cj1349, ciaB, and tlyA genes, 39/40 strains positive for the pldA gene, and 40/40 strains positive for the mviN gene. None of the strains presented the irgA gene. Hemagglutinin (hecA gene) and hemolysin (hecB) genes were detected in 21/40 and 16/40 strains, respectively. The SE-AFLP showed a great genetic diversity, but some clonally groups were disseminated in various tanks. These data reveal that the strains presented the same virulence traits described from Arcobacter isolated from food-borne disease in humans.
本研究的目的是对从巴西一个养殖场饲养的凯门鳄（Caiman yacare）中分离出的嗜低温嗜水气单胞菌和布氏嗜水气单胞菌进行鉴定和分子特征分析。分析了40份粪便样本。分离鉴定后，对40株中的21株布氏嗜水气单胞菌和19株嗜低温嗜水气单胞菌进行PCR检测潜在毒力基因。PCR结果显示，40株中有38株cadF、cj1349、ciaB和tlyA基因呈阳性，40株中有39株pldA基因呈阳性，40株中有40株mviN基因呈阳性。所有菌株均未呈现irgA基因。分别在40株中的21株和16株中检测到血凝素（hecA基因）和溶血素（hecB）基因。SE-AFLP显示出很大的遗传多样性，但一些克隆群在不同的养殖池中传播。这些数据表明，这些菌株具有与从人类食源性疾病中分离出的嗜水气单胞菌所描述的相同毒力特征。
