Oliveira Maria Gabriela Xavier de, Gomes Vasco Túlio de Moura, Cunha Marcos Paulo Vieira, Moreno Luisa Zanolli, Moreno Andrea Micke, Knöbl Terezinha
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo , São Paulo, Brazil .
Foodborne Pathog Dis. 2018 May;15(5):293-299. doi: 10.1089/fpd.2017.2368. Epub 2018 Feb 6.
Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii are Gram-negative pathogenic microorganisms that cause watery diarrhea and septicemia in humans. The aims of this study were to detect the presence of Arcobacter spp. in chicken meat from butcher shops in São Paulo (Brazil) and to verify their virulence genes and genotypic profiles. A total of 300 chicken cuts were analyzed. The results show the presence of Arcobacter spp. in 18.3% of samples, which were identified as A. butzleri (63.6%) and A. cryaerophilus (36.3%). All strains were positive for the virulence genes ciaB and mviN, followed by cj1349 (98%), pldA (94.4%), cadF (72.7%), tlyA (92.7%), hecA (49%), irgA (47.2%), and hecB (34.5%). These strains were subjected to single-enzyme amplified fragment length polymorphism. Nineteen genotypic profiles were obtained for A. butzleri, and 17 for A. cryaerophilus. These results confirm the presence of virulent strains of A. butzleri and A. cryaerophilus in the chicken meat in Brazil. The presence of potentially virulent strains of Arcobacter highlights a possible public health risk, particularly with respect to ingestion of undercooked foods and cross-contamination from uncooked foods during food preparation and contaminated utensils.
布氏嗜冷栖菌、嗜低温嗜冷栖菌和斯氏嗜冷栖菌是革兰氏阴性致病微生物，可导致人类出现水样腹泻和败血症。本研究的目的是检测圣保罗（巴西）肉铺鸡肉中嗜冷栖菌属的存在情况，并验证其毒力基因和基因型谱。共分析了300份鸡肉切块。结果显示，18.3%的样本中存在嗜冷栖菌属，其中鉴定为布氏嗜冷栖菌（63.6%）和嗜低温嗜冷栖菌（36.3%）。所有菌株的毒力基因ciaB和mviN均呈阳性，其次是cj1349（98%）、pldA（94.4%）、cadF（72.7%）、tlyA（92.7%）、hecA（49%）、irgA（47.2%）和hecB（34.5%）。这些菌株进行了单酶扩增片段长度多态性分析。布氏嗜冷栖菌获得了19种基因型谱，嗜低温嗜冷栖菌获得了17种。这些结果证实了巴西鸡肉中存在布氏嗜冷栖菌和嗜低温嗜冷栖菌的有毒菌株。嗜冷栖菌潜在有毒菌株的存在凸显了可能的公共卫生风险，特别是在摄入未煮熟的食物以及在食品制备过程中未煮熟食物和受污染器具的交叉污染方面。
