钩端螺旋体属在英国患有复发性葡萄膜炎马匹中的作用。
The role of Leptospira spp. in horses affected with recurrent uveitis in the UK.
作者信息
Malalana F, Blundell R J, Pinchbeck G L, Mcgowan C M
机构信息
School of Veterinary Science, University of Liverpool, Neston, UK.
Institute of Infection and Global Health, School of Veterinary Science, University of Liverpool, Neston, UK.
出版信息
Equine Vet J. 2017 Nov;49(6):706-709. doi: 10.1111/evj.12683. Epub 2017 Apr 24.
BACKGROUND
Equine recurrent uveitis (ERU) is a common cause of ocular pain and blindness in horses. Leptospira spp. have been commonly implicated in the pathophysiology of ERU in mainland Europe and the USA. No recent studies have been carried out in the UK, but Leptospira is reported not to be a major factor in the aetiology of ERU in the UK.
OBJECTIVES
To establish the prevalence of Leptospira-associated ERU in the UK and to identify the serovars involved in these cases; to compare serum vs. aqueous humour antibody levels in cases and controls in order to confirm the diagnosis of Leptospira-associated ERU, and to assess the usefulness of serology alone as a confirmatory test for Leptospira-associated ERU in the UK.
STUDY DESIGN
Case-control study.
METHODS
Eyes enucleated for clinical reasons in ERU-affected horses were collected. Blood and aqueous humour were obtained to determine antibody levels against a variety of Leptospira serovars and C-values (aqueous humour value/serum value) were calculated. In addition, eyes, blood and aqueous humour were obtained from control cases for comparison. Histopathology was performed in all eyes to confirm uveitis in each case. Differences in seroprevalences between ERU and control cases and between Leptospira- and non-Leptospira-associated ERU cases were calculated.
RESULTS
A total of 30 ERU and 43 control eyes were analysed. Of the ERU eyes, only two had a C-value of >4 (prevalence of Leptospira-associated uveitis: 6.7%). Serovars hardjo and javanica were detected. There was no difference in seroprevalence between horses with uveitis and control cases (65.5% and 41.9%, respectively; P = 0.11) or between Leptospira- and non-Leptospira-associated uveitis cases (100% and 63.0%, respectively; P = 0.52).
MAIN LIMITATIONS
The study was limited by low case numbers. Eyes were presented at different stages of disease. The only test used to detect Leptospira was the microscopic agglutination test.
CONCLUSIONS
Leptospira-associated ERU is uncommon in the UK. Serology alone may not help to definitively diagnose Leptospira-associated uveitis in this country.
背景
马复发性葡萄膜炎（ERU）是马匹眼部疼痛和失明的常见原因。在欧洲大陆和美国，钩端螺旋体属通常与ERU的病理生理学有关。英国最近没有进行相关研究，但据报道钩端螺旋体不是英国ERU病因的主要因素。
目的
确定英国钩端螺旋体相关ERU的患病率，并确定这些病例中涉及的血清型；比较病例组和对照组血清与房水抗体水平，以确诊钩端螺旋体相关ERU，并评估仅依靠血清学作为英国钩端螺旋体相关ERU确诊试验的有效性。
研究设计
病例对照研究。
方法
收集因临床原因摘除的患ERU马匹的眼睛。采集血液和房水以测定针对多种钩端螺旋体血清型的抗体水平，并计算C值（房水值/血清值）。此外，从对照病例中获取眼睛、血液和房水进行比较。对所有眼睛进行组织病理学检查以确认每例葡萄膜炎。计算ERU病例与对照病例之间以及钩端螺旋体相关和非钩端螺旋体相关ERU病例之间血清阳性率的差异。
结果
共分析了30只患ERU的眼睛和43只对照眼睛。在患ERU的眼睛中，只有两只C值>4（钩端螺旋体相关葡萄膜炎的患病率：6.7%）。检测到hardjo和javanica血清型。葡萄膜炎马匹与对照病例之间的血清阳性率无差异（分别为65.5%和41.9%；P = 0.11），钩端螺旋体相关和非钩端螺旋体相关葡萄膜炎病例之间也无差异（分别为100%和63.0%；P = 0.52）。
主要局限性
该研究受病例数量少的限制。眼睛处于疾病的不同阶段。用于检测钩端螺旋体的唯一试验是显微镜凝集试验。
结论
钩端螺旋体相关ERU在英国并不常见。仅依靠血清学可能无法明确诊断该国的钩端螺旋体相关葡萄膜炎。
Zotero 插件