Wang Yang, Liu Yongxia, Wan Huiyu, Zhu Yiran, Chen Peng, Hao Pan, Cheng Ziqiang, Liu Jianzhu
College of Veterinary Medicine, Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai`an, Shandong, 271018, People's Republic of China.
Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai`an, Shandong, 271018, People's Republic of China.
J Biochem Mol Toxicol. 2017 Aug;31(8). doi: 10.1002/jbt.21916. Epub 2017 Mar 21.
This study aimed to clarify the effect of selenium (Se) on chromium (VI) [Cr(VI)]-induced damage in chicken liver. A total of 105 chickens were randomly divided into seven groups of 15. Group I received deionized water; group II received Cr(VI) (7.83 mg/kg/d) alone; and other groups orally received both Cr(VI) (7.83 mg/kg/d) and Se of different doses (0.14, 0.29, 0.57, 1.14, and 2.28 mg/kg/d). The levels of superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), Ca -ATPase, and mitochondrial membrane potential (MMP) were measured. Results showed that Cr(VI) increased MDA content and decreased GSH content, T-SOD activity, Ca -ATPase activity, and MMP level. Meanwhile, Se co-treatment (0.14, 0.29, and 0.57 mg/kg/d) increased the viability of the above indicators compared with Cr(VI)-treatment alone. In addition, histopathologic examination revealed that Cr(VI) can cause liver damage, whereas Se supplementation of moderate dose inhibited this damage. This study confirmed that Se exerted protective effect against Cr(VI)-induced liver damage.
本研究旨在阐明硒（Se）对六价铬[Cr(VI)]诱导的鸡肝脏损伤的影响。总共105只鸡被随机分为7组，每组15只。第一组给予去离子水；第二组单独给予Cr(VI)（7.83毫克/千克/天）；其他组口服给予Cr(VI)（7.83毫克/千克/天）和不同剂量的Se（0.14、0.29、0.57、1.14和2.28毫克/千克/天）。测量超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、丙二醛（MDA）、Ca-ATP酶和线粒体膜电位（MMP）的水平。结果表明，Cr(VI)增加了MDA含量，降低了GSH含量、总SOD活性、Ca-ATP酶活性和MMP水平。同时，与单独Cr(VI)处理相比，联合使用Se（0.14、0.29和0.57毫克/千克/天）提高了上述指标的活力。此外，组织病理学检查显示，Cr(VI)可导致肝脏损伤，而补充中等剂量的Se可抑制这种损伤。本研究证实，Se对Cr(VI)诱导的肝脏损伤具有保护作用。
