Soudani Nejla, Ben Amara Ibtissem, Sefi Mediha, Boudawara Tahia, Zeghal Najiba
Animal Physiology Laboratory, UR/08-73 Sfax Faculty of Sciences, BP1171, 3000 Sfax, Tunisia.
Exp Toxicol Pathol. 2011 Sep;63(6):541-8. doi: 10.1016/j.etp.2010.04.005. Epub 2010 May 21.
Chromium, a major environmental pollutant, is known for its wide toxic manifestations. The present experiment pertains to the protective role of selenium (Se) against K(2)Cr(2)O(7)-induced hepatotoxicity. Female Wistar rats were divided into four groups of six each: group I served as controls which received standard diet; group II received in drinking water K(2)Cr(2)O(7) alone (700 ppm); group III received both K(2)Cr(2)O(7) and Se (0.5 Na(2)SeO(3) mg/kg of diet); group IV received Se (0.5 mg/kg of diet) for 3 weeks. Exposure of rats to chromium promoted oxidative stress with an increase in malondialdehyde (MDA) and a decrease in glutathione (GSH) levels. A decrease in glutathione peroxidase (GPx) and an increase in superoxide dismutase (SOD) and catalase (CAT) activities were observed. Se supplementation to the diet of group III improved all the parameters cited above. Yet, plasma transaminases (AST and ALT), lactate dehydrogenase (LDH) activities, cholesterol, triglycerides (TG) and low density lipoprotein-cholesterol (LDL-C) levels increased, while high density lipoprotein-cholesterol (HDL-C) decreased. Co-administration of Se to the diet of group III restored hepatic markers to near-normal values. The biochemical results confirmed the histopathological findings. Therefore, our investigation revealed that Se was effective in preventing K(2)Cr(2)O(7)-induced hepatotoxicity.
铬是一种主要的环境污染物，以其广泛的毒性表现而闻名。本实验探讨了硒（Se）对重铬酸钾（K₂Cr₂O₇）诱导的肝毒性的保护作用。将雌性Wistar大鼠分为四组，每组六只：第一组作为对照组，给予标准饮食；第二组仅在饮用水中给予K₂Cr₂O₇（700 ppm）；第三组给予K₂Cr₂O₇和Se（0.5 mg/kg饮食的亚硒酸钠）；第四组给予Se（0.5 mg/kg饮食），持续3周。大鼠接触铬会促进氧化应激，导致丙二醛（MDA）增加，谷胱甘肽（GSH）水平降低。观察到谷胱甘肽过氧化物酶（GPx）活性降低，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性增加。第三组饮食中补充Se改善了上述所有参数。然而，血浆转氨酶（AST和ALT）、乳酸脱氢酶（LDH）活性、胆固醇、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）水平升高，而高密度脂蛋白胆固醇（HDL-C）降低。第三组饮食中同时给予Se可使肝脏标志物恢复到接近正常的值。生化结果证实了组织病理学 findings。因此，我们的研究表明Se能有效预防K₂Cr₂O₇诱导的肝毒性。
