Goodwin Katharine, Nelson Celeste M
Department of Chemical & Biological Engineering, Princeton University, Princeton, NJ 08544, United States.
Department of Chemical & Biological Engineering, Princeton University, Princeton, NJ 08544, United States; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, United States.
Exp Cell Res. 2017 Sep 1;358(1):45-51. doi: 10.1016/j.yexcr.2017.03.016. Epub 2017 Mar 18.
During tissue morphogenesis, cellular rearrangements give rise to a large variety of three-dimensional structures. Final tissue architecture varies greatly across organs, and many develop to include combinations of folds, tubes, and branched networks. To achieve these different tissue geometries, constituent cells must follow different programs that dictate changes in shape and/or migratory behavior. One essential component of these changes is the remodeling of cell-cell adhesions. Invasive migratory behavior and separation between tissues require localized breakdown of cadherin-mediated adhesions. Conversely, tissue folding and fusion require the formation and reinforcement of cell-cell adhesions. Cell-cell adhesion plays a critical role in tissue morphogenesis; its manipulation may therefore prove to be invaluable in generating complex topologies ex vivo. Recapitulating these shapes in engineered tissues would enable a better understanding of how these processes occur in vivo, and may lead to improved design of organs for clinical applications. In this review, we discuss work investigating the formation of folds, tubes, and branched networks with an emphasis on known or possible roles for cell-cell adhesion. We then examine recently developed tools that could be adapted to manipulate cell-cell adhesion in engineered tissues.
在组织形态发生过程中，细胞重排产生了各种各样的三维结构。最终的组织结构在不同器官之间差异很大，许多器官发育成包括褶皱、管道和分支网络的组合。为了实现这些不同的组织几何形状，组成细胞必须遵循不同的程序，这些程序决定了形状和/或迁移行为的变化。这些变化的一个重要组成部分是细胞间粘附的重塑。侵袭性迁移行为和组织分离需要钙粘蛋白介导的粘附的局部破坏。相反，组织折叠和融合需要细胞间粘附的形成和加强。细胞间粘附在组织形态发生中起关键作用；因此，对其进行操控可能在体外生成复杂拓扑结构方面具有巨大价值。在工程组织中重现这些形状将有助于更好地理解这些过程在体内是如何发生的，并可能导致临床应用器官的设计改进。在这篇综述中，我们讨论了研究褶皱、管道和分支网络形成的工作，重点是细胞间粘附的已知或可能作用。然后，我们研究了最近开发的可用于操控工程组织中细胞间粘附的工具。
