Ventura M, Gibaud A, Le Pendu J, Hillaire D, Gérard G, Vitrac D, Oriol R
Institut d'Immunobiologie, CNRS ER-281, Hôpital Broussais, Paris, France.
Hum Hered. 1988;38(1):36-43. doi: 10.1159/000153752.
A simple method for the preparation of lymphoblastoid cell lines from small amounts (100 microliter) of frozen whole blood is described. A success score greater than 90% was obtained for EBV transformations using blood samples which had been collected several months before the infection. Due to the simplicity of the technique, up to 80 samples could be processed per day. This technique was used to prepared 242 permanent cell lines from 13 large families from Réunion Island showing blood group H deficiency. These cell lines are now available for genetic studies.
本文描述了一种从少量（100微升）冷冻全血制备淋巴母细胞系的简单方法。使用感染前几个月采集的血样进行EBV转化，成功率超过90%。由于该技术简单，每天可处理多达80个样本。该技术用于从留尼汪岛13个显示血型H缺乏的大家庭中制备了242个永久细胞系。这些细胞系现已可用于遗传学研究。
