Cameron Hamish, Campion Sylke H, Singh Tripti, Vaidya Alankar A
Faculty of Science and Engineering, University of Waikato, Private Bag 3105, Hamilton, New Zealand.
Scion, Te Papa Tipu Innovation Park, Private Bag 3020, Rotorua, 3046, New Zealand.
3 Biotech. 2015 Apr;5(2):221-225. doi: 10.1007/s13205-014-0212-2. Epub 2014 Apr 6.
Commercially available enzymes do not contain all the necessary softwood-specific accessory enzymes to obtain high saccharification efficiency. In this work, six saprophytic fungi obtained from Pinus radiata plantation site were screened for the putative softwood-specific accessory enzyme, β-mannanase. A Penicillium sp. was found to produce β-mannanase in both solid (31.6 units/g of dry biomass) and liquid (117 units/g of dry biomass) cultures using locust bean gum as an inducer after 2 weeks of incubation. The saccharification of steam-exploded Pinus radiata was 7.8 % w/w improved when the crude extract of Penicillium sp. was added to a mixture of commercial enzymes.
市售酶并不包含所有获得高糖化效率所需的软木特异性辅助酶。在这项工作中，对从辐射松人工林地点获得的六种腐生真菌进行了筛选，以寻找假定的软木特异性辅助酶——β-甘露聚糖酶。发现一种青霉菌株在以刺槐豆胶作为诱导剂的固体（31.6单位/克干生物质）和液体（117单位/克干生物质）培养物中，经过2周培养后可产生β-甘露聚糖酶。当将青霉菌株的粗提取物添加到商业酶混合物中时，蒸汽爆破处理的辐射松的糖化率提高了7.8%（重量/重量）。
