Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan, ROC.
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan, ROC.
J Colloid Interface Sci. 2017 Jul 15;498:144-152. doi: 10.1016/j.jcis.2017.03.045. Epub 2017 Mar 10.
To date, the natural alkaloids are mostly used in the field of pharmacological applications and the active substance of palmatine was extensively used in cancer therapy and other biomedical applications. Hence, in this study we report a simple preparation of poly-l-lysine (PLL) electro-polymerized on the surface of functionalized multiwalled carbon nanotubes (f-MWCNT) for electrochemical detection of palmatine content in human serum and urine samples. The active amino group of PLL plays a vital role towards the oxidation palmatine and exhibits superior electrocatalytic activity. Under optimum conditions, the prepared f-MWCNT/PLL composite shows a wide linear response range over the palmatine concentration ranging from 0.5µM to 425µM, and a detection limit (LOD) of 0.12µM based on S/N =3 (signal to noise ratio). The real time monitoring of palmatine content in serum and urine samples displays an appropriate recoveries and excellent performance for the practical analysis. The advantage of this developed system was simple, higher electrocatalytic activity, long-term stability and low cost. We hope that the prepared composite opens a new way for the fabrication of different biosensors in the field of biomedical application.
迄今为止，天然生物碱主要应用于药理学领域，小檗碱的活性物质广泛应用于癌症治疗和其他生物医学应用。因此，在本研究中，我们报告了一种简单的方法，即在功能化多壁碳纳米管（f-MWCNT）表面上聚合聚-l-赖氨酸（PLL），用于电化学检测人血清和尿液样品中的小檗碱含量。PLL 中的活性氨基基团对小檗碱的氧化起着至关重要的作用，并表现出优异的电催化活性。在最佳条件下，制备的 f-MWCNT/PLL 复合材料在小檗碱浓度为 0.5µM 至 425µM 的范围内表现出宽线性响应范围，基于 S/N=3（信号与噪声比）的检测限（LOD）为 0.12µM。实时监测血清和尿液样品中小檗碱的含量显示出适当的回收率和出色的实际分析性能。该开发系统的优点是简单、更高的电催化活性、长期稳定性和低成本。我们希望所制备的复合材料为生物医学应用领域的不同生物传感器的制造开辟新途径。
