Hixon Katherine R, Lu Tracy, McBride-Gagyi Sarah H, Janowiak Blythe E, Sell Scott A
Department of Biomedical Engineering, Parks College of Engineering, Aviation, and Technology, Saint Louis University, St. Louis, MO, USA.
Department of Orthopaedic Surgery, Saint Louis University, St. Louis, MO, USA.
Biomed Res Int. 2017;2017:4843065. doi: 10.1155/2017/4843065. Epub 2017 Feb 23.
. Manuka honey (MH) is an antibacterial agent specific to the islands of New Zealand containing both hydrogen peroxide and a Unique Manuka Factor (UMF). Although the antibacterial properties of MH have been studied, the effect of varying UMF of MH incorporated into tissue engineered scaffolds have not. Therefore, this study was designed to compare silk fibroin cryogels and electrospun scaffolds incorporated with a 5% MH concentration of various UMF. . Characteristics such as porosity, bacterial clearance and adhesion, and cytotoxicity were compared. . Pore diameters for all cryogels were between 51 and 60 m, while electrospun scaffolds were 10 m. Cryogels of varying UMF displayed clearance of approximately 0.16 cm for and . In comparison, the electrospun scaffolds clearance ranged between 0.5 and 1 cm. A glucose release of 0.5 mg/mL was observed for the first 24 hours by all scaffolds, regardless of UMF. With respect to cytotoxicity, neither scaffold caused the cell number to drop below 20,000. . Overall, when comparing the effects of the various UMF within the two scaffolds, no significant differences were observed. This suggests that the fabricated scaffolds in this study displayed similar bacterial effects regardless of the UMF value.
麦卢卡蜂蜜（MH）是一种新西兰岛屿特有的抗菌剂，含有过氧化氢和独特麦卢卡因子（UMF）。尽管已经对MH的抗菌特性进行了研究，但将不同UMF的MH掺入组织工程支架中的效果尚未研究。因此，本研究旨在比较掺入5%不同UMF浓度MH的丝素蛋白冷冻凝胶和电纺支架。比较了孔隙率、细菌清除和粘附以及细胞毒性等特性。所有冷冻凝胶的孔径在51至60μm之间，而电纺支架为10μm。不同UMF的冷冻凝胶对[具体细菌名称1]和[具体细菌名称2]的清除率约为0.16cm。相比之下，电纺支架的清除率在0.5至1cm之间。所有支架在前24小时均观察到0.5mg/mL的葡萄糖释放，与UMF无关。关于细胞毒性，两种支架均未导致细胞数量降至20000以下。总体而言，比较两种支架内不同UMF的效果时，未观察到显著差异。这表明本研究中制备的支架无论UMF值如何，均表现出相似的细菌作用效果。
