Department of Pathophysiology, Sun YatSen University, Guangzhou, Peoples Republic of China.
Cell Biol Int. 2010 Nov;34(11):1063-8. doi: 10.1042/CBI20090126.
To improve the survival and/or differentiation of grafted BMSCs (bone marrow stem cells) represents one of the challenges for the promising cell-based therapy. Considerable reports have implicated Sal B (salvianolic acid B), a potent aqueous extract of Salvia miltiorrhiza, in enhancing the survival of cells under various conditions. In this study, we investigated the effect of Sal B on H₂O₂-induced apoptosis in rat BMSCs, focusing on the survival signalling pathways. Results indicated that the MEK [MAPK (mitogen-activated protein kinase)/ERK (extracellular-signal-regulated kinase) kinase] inhibitor (PD98059) and 10 μM Sal B remarkably prevented BMSCs from H₂O₂-induced apoptosis through attenuating caspase-3 activation, which is accompanied by the significant up-regulation of Bcl-2. In addition, the ROS (reactive oxygen species) accumulation was also reduced after Sal B treatment. Furthermore, Sal B inhibited the ERK1/2 phosphorylations stimulated by H₂O₂. Taken together, our results showed that H₂O₂-induced apoptosis in BMSCs via the ROS/MEK/ERK1/2 pathway and Sal B may exert its cytoprotection through mediating the pathway.
提高移植骨髓间充质干细胞（BMSCs）的存活率和/或分化率是细胞治疗有前途的方向之一。有大量报道表明，丹参中的有效水溶性成分丹参素 B（Sal B）在各种条件下增强细胞存活率方面具有重要作用。在这项研究中，我们研究了 Sal B 对 H₂O₂诱导的大鼠 BMSCs 凋亡的影响，重点研究了存活信号通路。结果表明，MEK [丝裂原活化蛋白激酶/细胞外信号调节激酶（ERK）激酶]抑制剂（PD98059）和 10 μM Sal B 通过减弱半胱天冬酶-3 的激活，显著阻止 BMSCs 发生 H₂O₂诱导的细胞凋亡，Bcl-2 的表达显著上调。此外，Sal B 处理后 ROS（活性氧）的积累也减少了。此外，Sal B 抑制了 H₂O₂刺激的 ERK1/2 磷酸化。总之，我们的研究结果表明，H₂O₂通过 ROS/MEK/ERK1/2 通路诱导 BMSCs 凋亡，Sal B 可能通过介导该通路发挥其细胞保护作用。
