Almarzouqi Faris, Rennekampff Hans-Oliver, Stromps Jan-Philipp, Alharbi Ziyad, Pallua Norbert
Department of Plastic Surgery, Hand Surgery and Burn Center, RWTH University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
Eur J Med Res. 2017 Mar 21;22(1):10. doi: 10.1186/s40001-017-0251-3.
Cancer cells are typically surrounded by stromal cells and embedded in extracellular matrix (ECM). The stromal compartment interacts with cancer cells to promote growth and metastasis. For decades, autologous fasciocutaneous flaps have been safely applied for breast reconstruction after mastectomy. In contrast, the safety of fat grafting (lipofilling) procedure has been under debate regarding the risk of cancer recurrence.
Harvested fat tissue (lipoaspirates) and dissected abdominal fat (DAF) were co-cultured with MCF-7 breast cancer cells. The vitality of MCF-7 cells was measured using AlamarBlue consecutively for 5 days. ECM degradation was determined by detection of matrix metalloproteinase-1 (MMP-1) expression in MCF-7 cells. Integrin α2 was measured by Western blot to assess the degree of adhesion and motility of MFC-7 cells.
The MCF-7 proliferation increased substantially when co-cultured with fat tissue. However, there was no significant difference between the proliferation stimulating effects of lipoaspirates and DAF. Similarly, MMP-1 protein expression was equally elevated in MCF-7 cells by both lipoaspirates and DAF. Importantly, MCF-7 cells showed an increased level of integrin α2 once co-cultured with either lipoaspirates or DAF.
Fat tissue increases the proliferation of MCF-7 cells in vitro. Our data suggest that lipoaspirates as well as DAF might possess a considerable potency to promote tumorigenic growth of breast cancer cells. Thus, clinical trials are needed to address the safety of lipofilling by breast reconstruction surgery after mastectomy.
癌细胞通常被基质细胞包围并嵌入细胞外基质（ECM）中。基质成分与癌细胞相互作用以促进生长和转移。几十年来，自体筋膜皮瓣已被安全地应用于乳房切除术后的乳房重建。相比之下，脂肪移植（脂肪填充）手术的安全性一直因癌症复发风险而备受争议。
将采集的脂肪组织（抽脂物）和解剖的腹部脂肪（DAF）与MCF - 7乳腺癌细胞共培养。连续5天使用阿拉玛蓝测定MCF - 7细胞的活力。通过检测MCF - 7细胞中基质金属蛋白酶 - 1（MMP - 1）的表达来确定ECM降解情况。通过蛋白质印迹法测量整合素α2以评估MFC - 7细胞的粘附和运动程度。
与脂肪组织共培养时，MCF - 7的增殖显著增加。然而，抽脂物和DAF的增殖刺激作用之间没有显著差异。同样，抽脂物和DAF均使MCF - 7细胞中的MMP - 1蛋白表达同样升高。重要的是，MCF - 7细胞一旦与抽脂物或DAF共培养，整合素α2水平就会升高。
脂肪组织在体外增加MCF - 7细胞的增殖。我们的数据表明，抽脂物以及DAF可能具有相当大的促进乳腺癌细胞致瘤性生长的能力。因此，需要进行临床试验以解决乳房切除术后乳房重建手术中脂肪填充的安全性问题。
