Hoffmann F M, Fresco L D, Hoffman-Falk H, Shilo B Z
Cell. 1983 Dec;35(2 Pt 1):393-401. doi: 10.1016/0092-8674(83)90172-1.
Two cloned Drosophila sequences, Dash and Dsrc, were previously isolated by hybridization to the viral oncogenes v-abl and v-src. We report that the Drosophila DNA sequences are more than 50% homologous to 700 base pairs of the vertebrate oncogenes that are essential for kinase activity of the v-abl and the v-src gene products. Alignment of the predicted amino acid sequences in the homologous regions shows amino acid sequence identities of 74% between Dash and the 5' portion of v-abl and of 54% between Dsrc and 3' portion of v-src. Comparison of the Drosophila and vertebrate sequences identifies amino acids that may be essential for the distinct functions of the c-src and the c-abl gene products and places the gene duplication event that has generated the two genes prior to the Chordate-Arthropod divergence.
先前通过与病毒癌基因v-abl和v-src杂交，分离出了两个克隆的果蝇序列Dash和Dsrc。我们报告称，果蝇DNA序列与脊椎动物癌基因的700个碱基对具有超过50%的同源性，这些碱基对对于v-abl和v-src基因产物的激酶活性至关重要。同源区域中预测的氨基酸序列比对显示，Dash与v-abl的5'部分之间的氨基酸序列同一性为74%，Dsrc与v-src的3'部分之间的氨基酸序列同一性为54%。果蝇和脊椎动物序列的比较确定了可能对c-src和c-abl基因产物的独特功能至关重要的氨基酸，并将产生这两个基因的基因复制事件置于脊索动物-节肢动物分化之前。
