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一种细胞DNA结合蛋白与猿猴病毒40 DNA复制起点之间的序列特异性相互作用。

Sequence-specific interactions between a cellular DNA-binding protein and the simian virus 40 origin of DNA replication.

作者信息

Traut W, Fanning E

机构信息

Institute for Biochemistry, Munich, Federal Republic of Germany.

出版信息

Mol Cell Biol. 1988 Feb;8(2):903-11. doi: 10.1128/mcb.8.2.903-911.1988.

DOI:10.1128/mcb.8.2.903-911.1988
PMID:2832743
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363222/
Abstract

The core origin of simian virus 40 (SV40) DNA replication is composed of a 64-base-pair sequence encompassing T-antigen-binding site II and adjacent sequences on either side. A 7-base-pair sequence to the early side of T-antigen-binding site II which is conserved among the papovavirus genomes SV40, BK, JC, and SA12 was recently shown to be part of a 10-base-pair sequence required for origin activity (S. Deb, A.L. DeLucia, C.-P. Baur, A. Koff, and P. Tegtmeyer, Mol. Cell. Biol. 6:1663-1670, 1986), but its functional role was not defined. In the present report, we have used gel retention assays to identify a monkey cell factor that interacts specifically with double-stranded DNA carrying this sequence and also binds to single-stranded DNA. DNA-protein complexes formed with extracts from primate cells are more abundant and display electrophoretic mobilities distinct from those formed with rodent cell extracts. The binding activity of the factor on mutant templates is correlated with the replication activity of the origin. The results suggest that the monkey cell factor may be involved in SV40 DNA replication.

摘要

猿猴病毒40（SV40）DNA复制的核心起始区域由一段64个碱基对的序列组成，该序列包含T抗原结合位点II以及其两侧的相邻序列。T抗原结合位点II早期的一段7个碱基对的序列，在乳头瘤病毒基因组SV40、BK、JC和SA12中保守，最近被证明是起始活性所需的10个碱基对序列的一部分（S. Deb、A.L. DeLucia、C.-P. Baur、A. Koff和P. Tegtmeyer，《分子细胞生物学》6:1663 - 1670，1986），但其功能作用尚未明确。在本报告中，我们使用凝胶滞留分析来鉴定一种猴细胞因子，它能与携带该序列的双链DNA特异性相互作用，并且也能与单链DNA结合。与灵长类细胞提取物形成的DNA - 蛋白质复合物更为丰富，且显示出与啮齿动物细胞提取物形成的复合物不同的电泳迁移率。该因子在突变模板上的结合活性与起始区域的复制活性相关。结果表明，猴细胞因子可能参与SV40 DNA复制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/b70dcc166e3a/molcellb00062-0405-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/ba77d188587b/molcellb00062-0401-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/ab9ca6fa7265/molcellb00062-0403-b.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/a7658bd5103b/molcellb00062-0404-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/b70dcc166e3a/molcellb00062-0405-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/ba77d188587b/molcellb00062-0401-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/bd0f06356850/molcellb00062-0402-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/739515989551/molcellb00062-0402-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/2e4465fe940f/molcellb00062-0403-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/ab9ca6fa7265/molcellb00062-0403-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/53f54aef3104/molcellb00062-0404-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/363222/b70dcc166e3a/molcellb00062-0405-a.jpg

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本文引用的文献

1
Trans-complementable copy-number mutants of plasmid ColE1.质粒ColE1的反式可互补拷贝数突变体
Nature. 1980 Jan 10;283(5743):216-8. doi: 10.1038/283216a0.
2
Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides.用于合成寡脱氧核糖核苷酸的亚磷酰胺方法的改进。
Nucleic Acids Res. 1983 May 11;11(9):2575-84. doi: 10.1093/nar/11.9.2575.
3
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
高变微卫星DNA的重组热点活性需要微卫星DNA结合蛋白。
Somat Cell Mol Genet. 1998 Jan;24(1):41-51. doi: 10.1007/BF02677494.
4
A novel cellular site-specific DNA-binding protein cooperates with the viral NS1 polypeptide to initiate parvovirus DNA replication.一种新型细胞位点特异性DNA结合蛋白与病毒NS1多肽协同作用，启动细小病毒DNA复制。
J Virol. 1997 Feb;71(2):1405-16. doi: 10.1128/JVI.71.2.1405-1416.1997.
5
An N-terminal deletion mutant of simian virus 40 (SV40) large T antigen oligomerizes incorrectly on SV40 DNA but retains the ability to bind to DNA polymerase alpha and replicate SV40 DNA in vitro.猿猴病毒40（SV40）大T抗原的N端缺失突变体在SV40 DNA上错误地寡聚，但保留了与DNA聚合酶α结合并在体外复制SV40 DNA的能力。
J Virol. 1996 Jun;70(6):3509-16. doi: 10.1128/JVI.70.6.3509-3516.1996.
6
Characterization and cloning of p11, a transrepressor of Drosophila melanogaster retrotransposon 1731.果蝇反转录转座子1731的反式阻遏物p11的特性鉴定与克隆
Nucleic Acids Res. 1995 Dec 25;23(24):5073-9. doi: 10.1093/nar/23.24.5073.
7
Mutation of the cyclin-dependent kinase phosphorylation site in simian virus 40 (SV40) large T antigen specifically blocks SV40 origin DNA unwinding.猴病毒40（SV40）大T抗原中细胞周期蛋白依赖性激酶磷酸化位点的突变特异性地阻断了SV40起始DNA的解旋。
J Virol. 1993 Aug;67(8):4992-5002. doi: 10.1128/JVI.67.8.4992-5002.1993.
8
Initiation of simian virus 40 DNA replication requires the interaction of a specific domain of human DNA polymerase alpha with large T antigen.猿猴病毒40（Simian virus 40）DNA复制的起始需要人类DNA聚合酶α的一个特定结构域与大T抗原相互作用。
Mol Cell Biol. 1993 Feb;13(2):809-20. doi: 10.1128/mcb.13.2.809-820.1993.
9
Binding of a sequence-specific single-stranded DNA-binding factor to the simian virus 40 core origin inverted repeat domain is cell cycle regulated.序列特异性单链DNA结合因子与猿猴病毒40核心起始点反向重复结构域的结合受细胞周期调控。
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Species-specific functional interactions of DNA polymerase alpha-primase with simian virus 40 (SV40) T antigen require SV40 origin DNA.DNA聚合酶α-引发酶与猿猴病毒40（SV40）T抗原的物种特异性功能相互作用需要SV40起始DNA。
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Nucleic Acids Res. 1983 Mar 11;11(5):1475-89. doi: 10.1093/nar/11.5.1475.
4
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.使用M13衍生载体的寡核苷酸定向诱变：在任何DNA片段中产生点突变的高效通用方法。
Nucleic Acids Res. 1982 Oct 25;10(20):6487-500. doi: 10.1093/nar/10.20.6487.
5
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J Virol. 1983 Apr;46(1):151-61. doi: 10.1128/JVI.46.1.151-161.1983.
8
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J Mol Biol. 1982 Apr 15;156(3):531-48. doi: 10.1016/0022-2836(82)90265-0.
9
Territorial limits and functional anatomy of the simian virus 40 replication origin.猿猴病毒40复制起点的区域界限和功能解剖结构。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):381-5. doi: 10.1073/pnas.79.2.381.
10
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳研究乳糖阻遏物-操纵基因相互作用的平衡与动力学
Nucleic Acids Res. 1981 Dec 11;9(23):6505-25. doi: 10.1093/nar/9.23.6505.