• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于合成寡脱氧核糖核苷酸的亚磷酰胺方法的改进。

Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides.

作者信息

Dörper T, Winnacker E L

出版信息

Nucleic Acids Res. 1983 May 11;11(9):2575-84. doi: 10.1093/nar/11.9.2575.

DOI:10.1093/nar/11.9.2575
PMID:6856470
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325909/
Abstract

The paper describes an improved method for the synthesis of oligodeoxyribonucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3'-morpholine-methoxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reactions during solid-phase synthesis can thus be performed with high efficiency and result in a high yield synthesis of long chain oligodeoxyribonucleotides.

摘要

本文描述了一种使用亚磷酰胺化学合成寡脱氧核糖核苷酸的改进方法。我们的方法依赖于新型亚磷酰胺中间体，即脱氧核糖核苷-3'-吗啉甲氧基膦。这些化合物极其稳定，易于纯化。因此，固相合成过程中的缩合反应可以高效进行，并能高产率合成长链寡脱氧核糖核苷酸。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f7e/325909/a63040a7ed16/nar00354-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f7e/325909/fb6926712c08/nar00354-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f7e/325909/a63040a7ed16/nar00354-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f7e/325909/fb6926712c08/nar00354-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f7e/325909/a63040a7ed16/nar00354-0078-a.jpg

相似文献

1
Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides.用于合成寡脱氧核糖核苷酸的亚磷酰胺方法的改进。
Nucleic Acids Res. 1983 May 11;11(9):2575-84. doi: 10.1093/nar/11.9.2575.
2
Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.寡脱氧核糖核苷酸的快速合成。IV. 通过磷酸三酯中间体改进寡脱氧核糖核苷酸的固相合成
Nucleic Acids Res. 1980 Mar 11;8(5):1081-96. doi: 10.1093/nar/8.5.1081.
3
Solid-phase synthesis of the self-complementary hexamer d(c7GpCpc7GpCpc7GpC) via the O-3'-phosphoramidite of 7-deaza-2'-deoxyguanosine.通过7-脱氮-2'-脱氧鸟苷的O-3'-亚磷酰胺固相合成自互补六聚体d(c7GpCpc7GpCpc7GpC)
Nucleic Acids Res. 1985 Feb 11;13(3):911-26. doi: 10.1093/nar/13.3.911.
4
Rapid synthesis of oligodeoxyribonucleotides. V. Further studies in solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.寡聚脱氧核糖核苷酸的快速合成。V. 通过磷酸三酯中间体进行寡聚脱氧核糖核苷酸固相合成的进一步研究。
Nucleic Acids Symp Ser. 1980(7):243-57.
5
Synthetic strategies and parameters involved in the synthesis of oligodeoxyribonucleotides according to the phosphoramidite method.根据亚磷酰胺法合成寡脱氧核糖核苷酸所涉及的合成策略和参数。
Curr Protoc Nucleic Acid Chem. 2001 May;Chapter 3:Unit 3.3. doi: 10.1002/0471142700.nc0303s00.
6
A new linkage for solid phase synthesis of oligodeoxyribonucleotides.一种用于寡脱氧核糖核苷酸固相合成的新连接方法。
Nucleic Acids Res. 1985 Apr 25;13(8):2979-87. doi: 10.1093/nar/13.8.2979.
7
A rapid internucleotide bond formation for the phosphotriester approach by use of new condensing reagents.通过使用新型缩合试剂，磷酸三酯法实现核苷酸间快速成键。
Nucleic Acids Symp Ser. 1984(15):93-6.
8
A rapid deprotection procedure for phosphotriester DNA synthesis.一种用于磷酸三酯DNA合成的快速脱保护程序。
Nucleic Acids Res. 1984 Sep 11;12(17):6853-9. doi: 10.1093/nar/12.17.6853.
9
Use of the lipophilic tert-butyldiphenylsilyl protecting group in synthesis and rapid separation of polynucleotides.亲脂性叔丁基二苯基硅烷基保护基团在多核苷酸合成及快速分离中的应用。
Biochemistry. 1978 Apr 4;17(7):1268-78. doi: 10.1021/bi00600a021.
10
The use of barium salts of protected deoxyribonucleoside-3' p-chlorophenyl phosphates for construction of oligonucleotides by the phosphotriester method: high-yield synthesis of dinucleotide blocks.通过磷酸三酯法使用受保护的脱氧核糖核苷-3'-对氯苯基磷酸钡盐构建寡核苷酸：二核苷酸模块的高产合成
Nucleic Acids Res. 1979 Dec 11;7(7):1955-64. doi: 10.1093/nar/7.7.1955.

引用本文的文献

1
A size analysis of the adenovirus replicon.腺病毒复制子的大小分析。
EMBO J. 1984 Feb;3(2):333-7. doi: 10.1002/j.1460-2075.1984.tb01807.x.
2
Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.聚合物载体寡核苷酸合成 XVIII：脱氧核苷的β-氰基乙基-N，N-二烷基氨基-/N-吗啉代亚磷酰胺用于 DNA 片段合成，简化最终产物的脱保护和分离
Nucleic Acids Res. 1984 Jun 11;12(11):4539-57. doi: 10.1093/nar/12.11.4539.
3
Filter disc supported oligonucleotide synthesis by the phosphite method.

本文引用的文献

1
Gene expression: chemical synthesis of E. coli ribosome binding sites and their use in directing the expression of mammalian proteins in bacteria.基因表达：大肠杆菌核糖体结合位点的化学合成及其在指导细菌中哺乳动物蛋白表达中的应用。
Nucleic Acids Res. 1982 Oct 25;10(20):6319-29. doi: 10.1093/nar/10.20.6319.
2
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
3
Synthesis of thymidine oligonucleotides by phosphite triester intermediates.
滤膜支持的亚磷酸酯法寡核苷酸合成。
Nucleic Acids Res. 1984 Dec 11;12(23):9137-42. doi: 10.1093/nar/12.23.9137.
4
Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.使用羟基苯并三唑活化的磷酸三酯中间体进行聚合物支持的DNA合成。
Nucleic Acids Res. 1984 Nov 26;12(22):8639-51. doi: 10.1093/nar/12.22.8639.
5
Chemical synthesis of oligonucleotides containing a free sulphydryl group and subsequent attachment of thiol specific probes.含有游离巯基的寡核苷酸的化学合成及硫醇特异性探针的后续连接。
Nucleic Acids Res. 1985 Jun 25;13(12):4485-502. doi: 10.1093/nar/13.12.4485.
6
Mechanistic studies on the phosphoramidite coupling reaction in oligonucleotide synthesis. I. Evidence for nucleophilic catalysis by tetrazole and rate variations with the phosphorus substituents.寡核苷酸合成中磷酰胺酯偶联反应的机理研究。I. 四唑亲核催化的证据以及磷取代基对反应速率的影响
Nucleic Acids Res. 1987 Feb 25;15(4):1729-43. doi: 10.1093/nar/15.4.1729.
7
Application of 2-cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite for in situ preparation of deoxyribonucleoside phosphoramidites and their use in polymer-supported synthesis of oligodeoxyribonucleotides.2-氰基乙基-N,N,N',N'-四异丙基亚磷酰胺在原位制备脱氧核糖核苷亚磷酰胺中的应用及其在聚合物支持的寡脱氧核苷酸合成中的用途。
Nucleic Acids Res. 1986 Sep 25;14(18):7391-403. doi: 10.1093/nar/14.18.7391.
8
Solid phase synthesis of oligoribonucleotides using o-nitrobenzyl protection of 2'-hydroxyl via a phosphite triester approach.通过亚磷酸三酯法利用邻硝基苄基对2'-羟基进行保护的寡核糖核苷酸的固相合成。
Nucleic Acids Res. 1986 Aug 11;14(15):6265-79. doi: 10.1093/nar/14.15.6265.
9
A sensitive and rapid gel retention assay for nuclear factor I and other DNA-binding proteins in crude nuclear extracts.一种用于检测粗核提取物中核因子I和其他DNA结合蛋白的灵敏且快速的凝胶滞留分析方法。
Nucleic Acids Res. 1986 Feb 11;14(3):1303-17. doi: 10.1093/nar/14.3.1303.
10
Synthesis of a trans-syn thymine dimer building block. Solid phase synthesis of CGTAT[t,s]TATGC.反式-顺式胸腺嘧啶二聚体构建模块的合成。CGTAT[t,s]TATGC的固相合成。
Nucleic Acids Res. 1988 Jun 10;16(11):5123-36. doi: 10.1093/nar/16.11.5123.
通过亚磷酸三酯中间体合成胸苷寡核苷酸。
J Am Chem Soc. 1976 Jun 9;98(12):3655-61. doi: 10.1021/ja00428a045.
4
Synthesis of oligoribonucleotides based on the facile cleavage of methyl phosphotriester intermediates.基于甲基磷酸三酯中间体的简便裂解合成寡核糖核苷酸。
J Am Chem Soc. 1977 May 11;99(10):3526-8. doi: 10.1021/ja00452a069.