DeFENS, Department of Food, Environmental, and Nutritional Sciences, Food Packaging Lab., University of Milan, via Celoria 2-I, 20133 Milan, Italy.
Department of Food Engineering, Faculty of Engineering, Izmir Institute of Technology, Gülbahçe Köyü, Urla, Izmir 35430, Turkey.
Sensors (Basel). 2022 Dec 21;23(1):46. doi: 10.3390/s23010046.
A new molecularly imprinted electrochemical sensor was proposed to determine 4,4'-methylene diphenyl diamine (MDA) using molecularly imprinted polymer-multiwalled carbon nanotubes modified glassy carbon electrode (MIP/MWCNTs/GCE). GCE was coated by MWCNTs (MWCNTs/GCE) because of their antifouling qualities and in order to improve the sensor sensitivity. To make the whole sensor, a polymeric film made up of chitosan nanoparticles was electrodeposited by the cyclic voltammetry method on the surface of MWCNTs/GCE in the presence of MDA as a template. Different parameters such as scan cycles, elution time, incubation time, molar ratio of template molecules to functional monomers, and pH were optimized to increase the performance of the MIP sensor. With a detection limit of 15 nM, a linear response to MDA was seen in the concentration range of 0.5-100 µM. The imprinting factor (IF) of the proposed sensor was also calculated at around 3.66, demonstrating the extremely high recognition performance of a MIP/MWCNT-modified electrode. Moreover, the sensor exhibited good reproducibility and selectivity. Finally, the proposed sensor was efficiently used to determine MDA in real samples with satisfactory recoveries ranging from 94.10% to 106.76%.
一种新型的分子印迹电化学传感器被提出，用于使用分子印迹聚合物-多壁碳纳米管修饰的玻碳电极（MIP/MWCNTs/GCE）来测定 4,4'-亚甲基二苯胺（MDA）。由于多壁碳纳米管（MWCNTs）具有抗污染的特性，并且为了提高传感器的灵敏度，玻碳电极（GCE）被 MWCNTs 覆盖（MWCNTs/GCE）。为了制作整个传感器，在存在 MDA 作为模板的情况下，通过循环伏安法在 MWCNTs/GCE 表面上电沉积由壳聚糖纳米粒子组成的聚合膜。优化了不同的参数，如扫描循环、洗脱时间、孵育时间、模板分子与功能单体的摩尔比以及 pH 值，以提高 MIP 传感器的性能。该传感器的检测限为 15 nM，在 0.5-100 µM 的浓度范围内对 MDA 有线性响应。所提出的传感器的印迹因子（IF）也计算为约 3.66，表明 MIP/MWCNT 修饰电极具有极高的识别性能。此外，该传感器表现出良好的重现性和选择性。最后，该传感器在实际样品中有效地用于测定 MDA，回收率在 94.10%至 106.76%之间，令人满意。
