Król Żaneta, Marycz Krzysztof, Kulig Dominika, Marędziak Monika, Jarmoluk Andrzej
Department of Animal Products Technology and Quality Management, The Faculty of Food Science, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 37/41, 51-630 Wroclaw, Poland.
Department of Environment Hygiene and Animal Welfare, The Faculty of Biology and Animal Science, Wrocław University of Environmental and Life Sciences, Chelmonskiego 38 C, 50-630 Wroclaw, Poland.
Int J Mol Sci. 2017 Mar 22;18(3):678. doi: 10.3390/ijms18030678.
The aim of the study was to investigate the effect of using direct electric current (DC) of 0, 200, and 400 mA for five minutes on the physiochemical properties, cytotoxicity, antibacterial, and antioxidant activity of sodium alginate hydrosols with different sodium chloride concentrations. The pH, oxidation-reduction potential (ORP), electrical conductivity (EC), and available chlorine concentration (ACC) were measured. The effect of sodium alginate hydrosols treated with DC on , , , , , , , , and RAW 264.7 and L929 cells was investigated. Subsequently, the antioxidant properties of hydrosols were evaluated by determining the scavenging ability of 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) and ferric reducing antioxidant power (FRAP). The results have shown that after applying 400 mA in hydrosol samples with 0.1% and 0.2% NaCl all tested bacteria were inactivated. The ACC concentration of C400 samples with NaCl was equal to 13.95 and 19.71 mg/L, respectively. The cytotoxicity analysis revealed that optimized electric field conditions and the addition of sodium chloride allow for the avoidance of toxicity effects on normal cells without disturbing the antibacterial effects. Due to the presence of oxidizing substances, the DPPH of variants treated with DC was lower than the DPPH of control samples.
本研究的目的是调查分别施加0、200和400 mA直流电5分钟对不同氯化钠浓度的海藻酸钠水溶胶的理化性质、细胞毒性、抗菌和抗氧化活性的影响。测量了pH值、氧化还原电位（ORP）、电导率（EC）和有效氯浓度（ACC）。研究了经直流电处理的海藻酸钠水溶胶对[此处原文缺失部分内容]、RAW 264.7和L929细胞的影响。随后，通过测定1,1-二苯基-2-苦基肼自由基（DPPH）清除能力和铁还原抗氧化能力（FRAP）来评估水溶胶的抗氧化性能。结果表明，在含0.1%和0.2% NaCl的水溶胶样品中施加400 mA电流后，所有测试细菌均被灭活。含NaCl的C400样品的ACC浓度分别等于13.95和19.71 mg/L。细胞毒性分析表明，优化的电场条件和氯化钠的添加可避免对正常细胞产生毒性作用，同时不干扰抗菌效果。由于存在氧化物质，经直流电处理的变体的DPPH低于对照样品的DPPH。
