Castillo Christian, Muñoz Lorena, Carrillo Ileana, Liempi Ana, Gallardo Christian, Galanti Norbel, Maya Juan Diego, Kemmerling Ulrike
Programa de Anatomía y Biología del Desarrollo, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Am J Reprod Immunol. 2017 Jul;78(1). doi: 10.1111/aji.12660. Epub 2017 Mar 22.
Trypanosoma cruzi and Toxoplasma gondii present, respectively, low and high congenital transmission rates. The placenta as an immune regulatory organ expresses TLRs, leading to the secretion of cytokines. Both parasites are recognized by TLR-2, TLR-4, and TLR-9. Here, we studied if the parasites induce differences in TLR protein expression, cytokine profiles, and whether receptor inhibition is related to parasite infection.
Placental tissue explants were infected ex vivo with each parasite, TLRs protein expression, cytokine profile and parasite infection were determined by Western blotting, ELISA and qPCR.
Trypanosoma cruzi and Toxoplasma gondii infection is related to TLR-2 and TLR-4/TLR-9, respectively. Trypanosoma cruzi elicits an increase in TNF-α, IL-1β, IL-6, IL-8 and IL-10 cytokine secretion whereas T. gondii only increases the secretion of IL-8.
The susceptibility of the placenta to each parasite is mediated partially by the innate immune response.
克氏锥虫和刚地弓形虫分别呈现低和高的先天性传播率。胎盘作为一个免疫调节器官表达Toll样受体（TLRs），从而导致细胞因子的分泌。这两种寄生虫均可被TLR-2、TLR-4和TLR-9识别。在此，我们研究了这些寄生虫是否会诱导TLR蛋白表达、细胞因子谱的差异，以及受体抑制是否与寄生虫感染有关。
胎盘组织外植体在体外被每种寄生虫感染，通过蛋白质印迹法、酶联免疫吸附测定法（ELISA）和定量聚合酶链反应（qPCR）测定TLRs蛋白表达、细胞因子谱和寄生虫感染情况。
克氏锥虫感染与TLR-2相关，而刚地弓形虫感染与TLR-4/TLR-9相关。克氏锥虫引发肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和白细胞介素-10（IL-10）细胞因子分泌增加，而刚地弓形虫仅增加IL-8的分泌。
胎盘对每种寄生虫的易感性部分由先天性免疫反应介导。
