DNA连接的计算机模拟：确定有利于重组分子形成的初始DNA浓度。
Computer simulation of DNA ligation: determination of initial DNA concentrations favouring the formation of recombinant molecules.
作者信息
Dardel F
机构信息
Laboratoire de Biochimie, UA240 CNRS, Palaiseau, France.
出版信息
Nucleic Acids Res. 1988 Mar 11;16(5):1767-78. doi: 10.1093/nar/16.5.1767.
Abstract
A computer program was used to simulate the dynamic process of a ligation of DNA fragments. More specifically, the influence of the initial DNA fragments lengths and concentrations on the relative abundance of the various end-products was systematically investigated. Depending on the nature of the DNA extremities (asymmetric or symmetric, dephosphorylated or not), sets of initial conditions could be found that optimized the yield of active recombinant molecules. These results can be directly used to increase the efficiency of the ligation step, in particular for the construction of cDNA or genomic libraries.
摘要
使用计算机程序模拟DNA片段连接的动态过程。更具体地说，系统地研究了初始DNA片段长度和浓度对各种终产物相对丰度的影响。根据DNA末端的性质（不对称或对称、是否去磷酸化），可以找到优化活性重组分子产量的初始条件集。这些结果可直接用于提高连接步骤的效率，特别是用于构建cDNA或基因组文库。
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