Al-Haddab Mohammed, Abduljabbar Amr, Somily Ali Mohammed
Departments of *Dermatology and †Microbiology, King Saud University, Riyadh, Saudi Arabia.
Dermatol Surg. 2017 Jul;43(7):967-970. doi: 10.1097/DSS.0000000000001091.
Hyaluronic acid (HA) gel fillers represent most soft tissue augmentation procedures currently used, because they have lower rates of complications compared with other materials. Many patients do not consume an entire syringe of filler but may require a retouch or intermittent augmentation after some time. The remaining material is commonly stored in a specific environment for reuse by the same patient.
There are an insufficient number of recommendations concerning the safety of storing and reusing dermal fillers in the literature because of the paucity of studies. The aim of this study was to investigate the potential infectious contamination associated with the storage of HA fillers after patient treatment.
Hyaluronic acid from previously used syringes was stored at room temperature under sterile conditions for varying durations beginning from 2009. Later, the material was submitted for panculture, including gram-positive and gram-negative bacteria, mycobacteria, and fungi.
No fungal or mycobacterial agents were cultured from any of the samples. There were a few positive bacterial cultures, but they were predominantly contaminated with normal skin surface flora.
Although it is commonly practiced, the storage of HA fillers after initial patient injection carries a real but small risk of contamination.
透明质酸（HA）凝胶填充剂是目前最常用的软组织填充材料，因为与其他材料相比，其并发症发生率较低。许多患者并未用完一整支填充剂，但一段时间后可能需要进行修补或间歇性填充。剩余的材料通常存放在特定环境中以供同一患者再次使用。
由于相关研究较少，文献中关于储存和重复使用真皮填充剂安全性的建议数量不足。本研究的目的是调查患者治疗后HA填充剂储存过程中潜在的感染性污染情况。
从2009年开始，将先前使用过的注射器中的透明质酸在无菌条件下于室温储存不同时长。之后，将材料送去进行全面培养，包括革兰氏阳性菌和革兰氏阴性菌、分枝杆菌和真菌。
所有样本均未培养出真菌或分枝杆菌病原体。有一些细菌培养呈阳性，但主要是被正常皮肤表面菌群污染。
尽管这是常见做法，但首次给患者注射后储存HA填充剂存在实际但较小的污染风险。
