Abinandan S, Shanthakumar S
Environmental Engineering Division, School of Mechanical and Building Sciences, VIT University, Vellore, 632014, India.
3 Biotech. 2016 Jun;6(1):9. doi: 10.1007/s13205-015-0314-5. Epub 2016 Jan 5.
Bicarbonate species in the aqueous phase is the primary source for CO for the growth of microalgae. The potential of carbon dioxide (CO) fixation by Chlorella pyrenoidosa in enriched bicarbonate medium was evaluated. In the present study, effects of parameters such as pH, sodium bicarbonate concentration and inoculum size were assessed for the removal of CO by C. pyrenoidosa under mixotrophic condition. Central composite design tool from response surface methodology was used to validate statistical methods in order to study the influence of these parameters. The obtained results reveal that the maximum removal of CO was attained at pH 8 with sodium bicarbonate concentration of 3.33 g/l, and inoculum size of 30 %. The experimental results were statistically significant with R value of 0.9527 and 0.960 for CO removal and accumulation of chlorophyll content, respectively. Among the various interactions, interactive effects between the parameters pH and inoculum size was statistically significant (P < 0.05) for CO removal and chlorophyll accumulation. Based on the studies, the application of C. pyrenoidosa as a potential source for carbon dioxide removal at alkaline pH from bicarbonate source is highlighted.
水相中的碳酸氢盐是微藻生长所需二氧化碳的主要来源。评估了在富含碳酸氢盐的培养基中，小球藻固定二氧化碳的潜力。在本研究中，评估了pH值、碳酸氢钠浓度和接种量等参数对混合营养条件下小球藻去除二氧化碳的影响。采用响应面法中的中心复合设计工具来验证统计方法，以研究这些参数的影响。所得结果表明，在pH值为8、碳酸氢钠浓度为3.33 g/l、接种量为30%时，二氧化碳的去除量最大。实验结果具有统计学意义，二氧化碳去除率和叶绿素含量积累的R值分别为0.9527和0.960。在各种相互作用中，pH值和接种量之间的交互作用对二氧化碳去除和叶绿素积累具有统计学意义（P < 0.05）。基于这些研究，强调了小球藻作为从碳酸氢盐源在碱性pH下去除二氧化碳的潜在来源的应用。
