Raghav Nishu, Singh Rajender, Chhokar Rajender Singh, Sharma Davinder, Kumar Raman
ICAR-Indian Institute of Wheat and Barley Research, Post Box 158, Karnal, 132001, India.
Maharishi Markandeshwar University, Mullana, India.
3 Biotech. 2016 Jun;6(1):12. doi: 10.1007/s13205-015-0331-4. Epub 2016 Jan 5.
Littleseed canarygrass (Phalaris minor Retz.) is one of the most common and troublesome weeds infesting wheat crop in India. Repeated use during the last two decades of the ACCase-inhibiting herbicide (clodinafop) to control this weed has resulted in the occurrence of resistance. Fifty-three P. minor populations were collected from wheat fields in Haryana and Punjab states of India. The dose-response assays indicated that 29 populations were resistant, 23 populations were susceptible and one population was moderately resistant to clodinafop. Sequence analysis of the CT domain of ACCase gene among resistant and susceptible populations revealed two non-synonymous mutations, Trp to Cys and Ile to Asn in the resistant populations. Allele-specific PCR markers were developed to differentiate between wild-type and resistant codons at positions 2027 and 2041 of ACCase in P. minor which enables molecular assays for rapid detection and resistance diagnosis for efficient weed management in wheat. This is the first report from India of a target site mutation corresponding to resistance to clodinafop in P. minor.
小粒雀麦（Phalaris minor Retz.）是印度小麦作物中最常见且最棘手的杂草之一。在过去二十年中，为控制这种杂草反复使用乙酰辅酶A羧化酶（ACCase）抑制性除草剂（氯氟吡氧乙酸）导致了抗性的出现。从印度哈里亚纳邦和旁遮普邦的麦田中采集了53个小粒雀麦种群。剂量反应试验表明，29个种群具有抗性，23个种群敏感，1个种群对氯氟吡氧乙酸具有中等抗性。抗性和敏感种群中ACCase基因CT结构域的序列分析显示，抗性种群中有两个非同义突变，即色氨酸突变为半胱氨酸以及异亮氨酸突变为天冬酰胺。开发了等位基因特异性PCR标记，以区分小粒雀麦ACCase第2027位和第2041位的野生型和抗性密码子，这使得能够进行分子检测，用于快速检测和抗性诊断，以实现小麦杂草的有效管理。这是印度首次报道小粒雀麦中与对氯氟吡氧乙酸抗性相关的靶标位点突变。
