Azmi Syed Afrin, Chatterjee Soumendranath
Parasitology and Microbiology Research Laboratory, Department of Zoology, The University of Burdwan, Burdwan, West Bengal, 713104, India.
3 Biotech. 2016 Jun;6(1):37. doi: 10.1007/s13205-015-0361-y. Epub 2016 Jan 23.
In this present study the population dynamics of the soil bacteria of some coastal villages, namely Padima, Jatimati, Chanpabani, Palsandapur, Bhagibaharampur, Duttapur, Gangadharpur, Gobindabasan, Somaibasan of Digha, West Bengal, India, was determined. In these villages the aerobic heterotrophic, Gram-negative, spore-forming, starch-hydrolyzing, Pseudomonas, nitrate-reducing, denitrifying, asymbiotic N fixing, nitrifying, phosphate-solubilizing bacterial populations ranged from 1.22 to 2.67 × 10, 0.09-1.63 × 10, 1.53-3.68 × 10, 2.22-4.06 × 10, 0.02-0.04 × 10, 0.35-1.33 × 10, 0.07-0.82 × 10, 0.58-2.50 × 10, 0.13-2.35 × 10, 0.05-1.9 × 10 cfu/g dry soil, respectively. The organic carbon content of the soil samples ranged from 0.61 to 0.93 %. The available nitrogen and phosphate in the soils of the study area varied from 11.2 to 29.5 and 230.8-503.09 mg/kg, respectively. The one-way ANOVA revealed significant variations (p < 0.05) in the microbial diversity with respect to different locations of the study site. Shannon-Wiener and Simpson Index of the study areas ranged from 1.56 to 1.88 and 3.85-5.73, respectively. Jatimati showed comparatively higher diversity index among the villages of the study area. From principal component analysis, three components were extracted having the Eigen values of 3.541, 1.603 and 1.391, respectively. Agglometric hierarchial cluster analysis in respect of the number of different bacterial groups in different places of the study area showed that the denitrifying, nitrate reducing, asymbiotic nitrogen-fixing and spore-forming bacteria formed a cluster while Pseudomonas differed from them forming another cluster and nitrifying, Gram negative, phosphate-solubilising and starch-hydrolyzing bacteria formed another different cluster. This variation of the soil bacteria might be dependent on the microhabitat present in different locations of the study area.
在本研究中，测定了印度西孟加拉邦迪加的一些沿海村庄，即帕迪马、贾蒂马蒂、钱帕巴尼、帕尔桑达布尔、巴吉巴哈兰布尔、杜塔布尔、甘加德哈布尔、戈宾达巴桑、索马伊巴桑土壤细菌的种群动态。在这些村庄中，需氧异养菌、革兰氏阴性菌、芽孢杆菌、淀粉水解菌、假单胞菌、硝酸盐还原菌、反硝化菌、非共生固氮菌、硝化菌、解磷菌的数量分别在1.22至2.67×10、0.09 - 1.63×10、1.53 - 3.68×10、2.22 - 4.06×10、0.02 - 0.04×10、0.35 - 1.33×10、0.07 - 0.82×10、0.58 - 2.50×10、0.13 - 2.35×10、0.05 - 1.9×10 cfu/g干土之间。土壤样品的有机碳含量在0.61%至0.93%之间。研究区域土壤中的有效氮和磷分别在11.2至29.5和230.8 - 503.09 mg/kg之间。单因素方差分析显示，研究地点不同位置的微生物多样性存在显著差异（p < 0.05）。研究区域的香农 - 维纳指数和辛普森指数分别在1.56至1.88和3.85 - 5.73之间。在研究区域的村庄中，贾蒂马蒂的多样性指数相对较高。通过主成分分析，提取了三个主成分特征值分别为3.541、1.603和1.391。对研究区域不同地点不同细菌类群数量的聚类层次分析表明，反硝化菌、硝酸盐还原菌、非共生固氮菌和芽孢杆菌形成一个聚类，而假单胞菌与它们不同，形成另一个聚类，硝化菌、革兰氏阴性菌、解磷菌和淀粉水解菌形成另一个不同的聚类。土壤细菌的这种变化可能取决于研究区域不同位置的微生境。
