Das Arpan, Ghosh Priyanka, Paul Tanmay, Ghosh Uma, Pati Bikas Ranjan, Mondal Keshab Chandra
Department of Microbiology, Maulana Azad College, Kolkata, West Bengal, 700013, India.
Food Technology and Biochemical Engineering Department, Jadavpur University, Kolkata, West Bengal, 700032, India.
3 Biotech. 2016 Jun;6(1):70. doi: 10.1007/s13205-016-0385-y. Epub 2016 Feb 15.
Water hyacinth (Eichhornia crassipes) represents a promising candidate for fuel ethanol production in tropical countries because of their high availability and high biomass yield. Bioconversion of such biomass to bioethanol could be wisely managed through proper technological approach. In this work, pretreatment of water hyacinth (10 %, w/v) with dilute sulfuric acid (2 %, v/v) at high temperature and pressure was integrated in the simulation and economic assessment of the process for further enzymatic saccharification was studied. The maximum sugar yield (425.6 mg/g) through enzymatic saccharification was greatly influenced by the solid content (5 %), cellulase load (30 FPU), incubation time (24 h), temperature (50 °C), and pH (5.5) of the saccharifying medium. Central composite design optimized an ethanol production of 13.6 mg/ml though a mixed fermentation by Saccharomyces cerevisiae (MTCC 173) and Zymomonas mobilis (MTCC 2428). Thus the experiment imparts an economic value to water hyacinths that are cleared from choking waterways.
凤眼蓝（Eichhornia crassipes）因其在热带国家易于获取且生物质产量高，成为燃料乙醇生产的理想原料。通过适当的技术方法，可以明智地管理这种生物质向生物乙醇的生物转化。在本研究中，将凤眼蓝（10%，w/v）在高温高压下用稀硫酸（2%，v/v）进行预处理，并将其纳入该过程的模拟和经济评估中，进而研究了进一步的酶促糖化。酶促糖化的最大糖产量（425.6 mg/g）受糖化培养基的固体含量（5%）、纤维素酶负载量（30 FPU）、孵育时间（24小时）、温度（50°C）和pH值（5.5）的显著影响。中心复合设计通过酿酒酵母（MTCC 173）和运动发酵单胞菌（MTCC 2428）的混合发酵，优化得到了13.6 mg/ml的乙醇产量。因此，该实验赋予了从堵塞水道中清除的凤眼蓝经济价值。
