Nongthombam Grihalaksmi Devi, Sarangi Prakash Kumar, Singh Thangjam Anand, Sharma Chandradev K, Talukdar Narayan C
Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal, 795001 India.
College of Agriculture, Central Agricultural University, Imphal, 795004 India.
3 Biotech. 2022 Sep;12(9):178. doi: 10.1007/s13205-022-03234-y. Epub 2022 Jul 18.
is among the few filamentous fungi capable of fermenting ethanol directly from lignocellulose biomass (LCB). It has the essential enzymatic toolbox to disintegrate LCB to its monosaccharides, which subsequently fermented to ethanol under anaerobic and micro-aerobic conditions. However, the structural complexity of LCB and modest performances of wild fungi are major limitations for application in local biorefineries. This study assessed the potential of the locally isolated for the production of bioethanol from fruits () using Consolidated Bioprocessing (CBP). The maximum ethanol concentration achieved was at 5% substrate loadings with pH 6 irrespective of temperature variance, attaining a concentration of 3.54 g/L and 3.88 g/L at 28 °C and 32 °C, respectively. The monitoring of analytes (glucose, arabinose, cellobiose, xylose, acetic acid, ethanol, furfural, and HMF) in this study suggests the utilization of an array of sugars released from fruits, irrespective of the difference in the process parameters. This study also shows that CBP of freshly grounded fruits was feasible employing a mild hydrothermal pretreatment (autoclaved at 121 °C for 30 min in 1:10 /) and without supplementing any extraneous enzymes.
The online version contains supplementary material available at 10.1007/s13205-022-03234-y.
是少数能够直接从木质纤维素生物质（LCB）发酵乙醇的丝状真菌之一。它拥有将LCB分解为单糖的基本酶工具箱，这些单糖随后在厌氧和微需氧条件下发酵成乙醇。然而，LCB的结构复杂性和野生真菌的适度性能是其在当地生物精炼厂应用的主要限制。本研究评估了本地分离的[具体名称未给出]利用综合生物加工（CBP）从[水果名称未给出]水果中生产生物乙醇的潜力。无论温度如何变化，在底物负载量为5%、pH值为6时达到的最大乙醇浓度，在28°C和32°C时分别达到3.54 g/L和3.88 g/L。本研究中对分析物（葡萄糖、阿拉伯糖、纤维二糖、木糖、乙酸、乙醇、糠醛和HMF）的监测表明，无论工艺参数有何差异，均利用了从[水果名称未给出]水果中释放的一系列糖类。本研究还表明，采用温和的水热预处理（在121°C下高压灭菌30分钟，固液比为1:10）且不添加任何外源酶的情况下，对新鲜研磨的[水果名称未给出]水果进行CBP是可行的。
在线版本包含可在10.1007/s13205-022-03234-y获取的补充材料。
