Bioethanol production from fruits () by through consolidated bioprocessing system.

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is among the few filamentous fungi capable of fermenting ethanol directly from lignocellulose biomass (LCB). It has the essential enzymatic toolbox to disintegrate LCB to its monosaccharides, which subsequently fermented to ethanol under anaerobic and micro-aerobic conditions. However, the structural complexity of LCB and modest performances of wild fungi are major limitations for application in local biorefineries. This study assessed the potential of the locally isolated for the production of bioethanol from fruits () using Consolidated Bioprocessing (CBP). The maximum ethanol concentration achieved was at 5% substrate loadings with pH 6 irrespective of temperature variance, attaining a concentration of 3.54 g/L and 3.88 g/L at 28 °C and 32 °C, respectively. The monitoring of analytes (glucose, arabinose, cellobiose, xylose, acetic acid, ethanol, furfural, and HMF) in this study suggests the utilization of an array of sugars released from fruits, irrespective of the difference in the process parameters. This study also shows that CBP of freshly grounded fruits was feasible employing a mild hydrothermal pretreatment (autoclaved at 121 °C for 30 min in 1:10 /) and without supplementing any extraneous enzymes.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-022-03234-y.