Cummins T J, Smith R E
Department of Microbiology, Colorado State University, Fort Collins 80523.
Virology. 1988 Apr;163(2):452-61. doi: 10.1016/0042-6822(88)90286-3.
Infection of 10-day-old chickens with an avian osteopetrosis virus resulted in a severe regenerative aplastic crisis. Hematopoietic and lymphopoietic tissues of chickens infected with myeloblastosis-associated virus (of subgroup B, inducing osteopetrosis, MAV-2(O] were analyzed for integrated and unintegrated viral DNA sequences, cell population shifts, weight changes, and morphological alterations. By 6 days postinfection (p.i.), DNA from bone marrow cells and peripheral blood leukocytes (PBL) contained between 0.50 and 0.70 copies of viral DNA per haploid genome. Erythrocytes and splenic leukocytes contained less than 0.10 copies/haploid genome. Granulocytes and precursor mesomyelocytes were absent from bone marrow, but numbers of erythrocytes, erythroblasts, and reticulocytes were normal. By 9 days p.i., bone marrow was severely hypoplastic and both granulopoietic and erythropoietic colonies were depleted. By 12 days p.i., erythrocytes and granulocytes were maximally depressed in peripheral blood and the amount of integrated virus in bone marrow and PBL decreased to less than 0.20 copies/haploid genome. In contrast, erythrocytes contained integrated viral DNA of up to 0.30 copies/haploid genome, indicating infection of erythrocyte precursors. At 18 days p.i., viral DNA was detected only in erythrocytes. Unintegrated viral DNA was not detected in any organs. Anemia was accompanied by splenomegaly and erythrophagocytosis. Viral DNA was never detected in thymus or bursa. Differential counting and flow cytometry of cells from bursa, thymus, and spleen, and of blood lymphocytes did not detect significant population shifts. These results suggest that MAV-2(O) infection of immunocompetent chickens occurs primarily in myelopoietic tissues, and tissues are selectively infected.
用禽骨质石化病毒感染10日龄鸡会导致严重的再生障碍性危象。对感染成髓细胞增多症相关病毒（B亚群，可诱导骨质石化，MAV-2[O]）的鸡的造血组织和淋巴细胞组织进行分析，检测其中整合和未整合的病毒DNA序列、细胞群体变化、体重变化以及形态学改变。感染后6天（p.i.），骨髓细胞和外周血白细胞（PBL）的DNA中每单倍体基因组含有0.50至0.70份病毒DNA拷贝。红细胞和脾白细胞中每单倍体基因组含有的病毒DNA拷贝数少于0.10份。骨髓中粒细胞和前体中髓细胞缺失，但红细胞、成红细胞和网织红细胞数量正常。感染后9天，骨髓严重发育不全，粒细胞生成和红细胞生成集落均减少。感染后12天，外周血中红细胞和粒细胞降至最低水平，骨髓和PBL中整合病毒的量降至每单倍体基因组少于0.20份拷贝。相比之下，红细胞中整合的病毒DNA高达每单倍体基因组0.30份拷贝，表明红细胞前体受到感染。感染后18天，仅在红细胞中检测到病毒DNA。在任何器官中均未检测到未整合的病毒DNA。贫血伴有脾肿大和红细胞吞噬现象。在胸腺或法氏囊中从未检测到病毒DNA。对来自法氏囊、胸腺和脾脏的细胞以及血液淋巴细胞进行细胞分类计数和流式细胞术分析，未检测到明显的细胞群体变化。这些结果表明，免疫活性鸡感染MAV-2[O]主要发生在骨髓组织，且组织受到选择性感染。
