Lim Mim Mim, Sultana Naznin
Department of Clinical Sciences, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Johor Bahru, 81310, Johor, Malaysia.
Advanced Membrane Technology research Center (AMTEC), Universiti Teknologi Malaysia, Johor Bahru, 81310, Johor, Malaysia.
3 Biotech. 2016 Dec;6(2):211. doi: 10.1007/s13205-016-0531-6. Epub 2016 Sep 29.
The development of nano-sized scaffolds with antibacterial properties that mimic the architecture of tissue is one of the challenges in tissue engineering. In this study, polycaprolactone (PCL) and PCL/gelatine (Ge) (70:30) nanofibrous scaffolds were fabricated using a less toxic and common solvent, formic acid and an electrospinning technique. Nanofibrous scaffolds were coated with silver (Ag) in different concentrations of silver nitrate (AgNO) aqueous solution (1.25, 2.5, 5, and 10 %) by using dipping method, drying and followed by ultraviolet (UV) photoreduction. The PCL/Ge (70:30) nanofibrous scaffold had an average fibre diameter of 155.60 ± 41.13 nm. Characterization showed that Ag was physically entrapped in both the PCL and PCL/Ge (70:30) nanofibrous scaffolds. Ag ions release study was performed and showed much lesser release amount than the maximum toxic concentration of Ag ions in human cells. Both scaffolds were non-toxic to cells and demonstrated antibacterial effects towards Gram-positive Bacillus cereus (B. cereus) and Gram-negative Escherichia coli (E. coli). The Ag/PCL/Ge (70:30) nanofibrous scaffold has potential for tissue engineering as it can protect wounds from bacterial infection and promote tissue regeneration.
开发具有抗菌特性且能模拟组织结构的纳米级支架是组织工程领域面临的挑战之一。在本研究中，使用毒性较小且常用的溶剂甲酸和静电纺丝技术制备了聚己内酯（PCL）和PCL/明胶（Ge）（70:30）纳米纤维支架。通过浸渍法，将纳米纤维支架用不同浓度（1.25%、2.5%、5%和10%）的硝酸银（AgNO₃）水溶液包覆银（Ag），干燥后进行紫外（UV）光还原。PCL/Ge（70:30）纳米纤维支架的平均纤维直径为155.60±41.13纳米。表征显示，Ag物理包埋在PCL和PCL/Ge（70:30）纳米纤维支架中。进行了银离子释放研究，结果表明释放量远低于银离子在人体细胞中的最大毒性浓度。两种支架对细胞均无毒，并对革兰氏阳性蜡样芽孢杆菌（B. cereus）和革兰氏阴性大肠杆菌（E. coli）具有抗菌作用。Ag/PCL/Ge（70:30）纳米纤维支架具有组织工程应用潜力，因为它可以保护伤口免受细菌感染并促进组织再生。
