Bacteriology and Mycology Laboratory, Department of Public Health and Pediatrics, University of Torino, Via Santena 9, 10126 Turin, Italy.
Immunology Laboratory, Department of Public Health and Pediatrics, University of Torino, Via Santena 9, 10126 Turin, Italy.
Int J Mol Sci. 2021 Sep 21;22(18):10176. doi: 10.3390/ijms221810176.
In this study, we designed and developed novel poly(ε-caprolactone) (PCL)-based biomaterials, for use as bone scaffolds, through modification with both biphasic calcium phosphate (BCP), to impart bioactive/bioresorbable properties, and with silver nitrate, to provide antibacterial protection against , a microorganism involved in prosthetic joint infections (PJIs). Field emission scanning electron microscopy (FESEM) showed that the samples were characterized by square-shaped macropores, and energy dispersive X-ray spectroscopy analysis confirmed the presence of PCL and BCP phases, while inductively coupled plasma-mass spectrometry (ICP-MS) established the release of Ag in the medium (~0.15-0.8 wt% of initial Ag content). Adhesion assays revealed a significant ( < 0.0001) reduction in both adherent and planktonic staphylococci on the Ag-functionalized biomaterials, and the presence of an inhibition halo confirmed Ag release from enriched samples. To assess the potential outcome in promoting bone integration, preliminary tests on sarcoma osteogenic-2 (Saos-2) cells indicated PCL and BCP/PCL biocompatibility, but a reduction in viability was observed for Ag-added biomaterials. Due to their combined biodegrading and antimicrobial properties, the silver-enriched BCP/PCL-based scaffolds showed good potential for engineering of bone tissue and for reducing PJIs as a microbial anti-adhesive tool used in the delivery of targeted antimicrobial molecules, even if the amount of silver needs to be tuned to improve osteointegration.
在这项研究中，我们通过用双相磷酸钙（BCP）对其进行改性，赋予其生物活性/生物可吸收特性，并用硝酸银提供抗菌保护，来设计和开发新型聚（ε-己内酯）（PCL）基生物材料，用作骨支架。BCP 可以赋予生物活性/生物可吸收特性，而硝酸银可以提供抗菌保护，防止引起假体关节感染（PJI）的微生物。场发射扫描电子显微镜（FESEM）显示，这些样品的特征是具有方形大孔，能谱分析（EDS）证实了 PCL 和 BCP 相的存在，而电感耦合等离子体质谱（ICP-MS）则确定了 Ag 在介质中的释放（~初始 Ag 含量的 0.15-0.8wt%）。粘附试验表明，在 Ag 功能化生物材料上，粘附和浮游葡萄球菌的数量均显著减少（<0.0001），并且存在抑制晕圈证实了从富集样品中释放了 Ag。为了评估促进骨整合的潜在结果，对骨肉瘤成骨-2 （Saos-2）细胞进行的初步测试表明 PCL 和 BCP/PCL 具有生物相容性，但添加 Ag 的生物材料的活力降低。由于其具有生物降解和抗菌性能，富含 Ag 的 BCP/PCL 基支架具有良好的工程骨组织的潜力，并可作为一种微生物抗粘附工具，用于输送靶向抗菌分子，以减少 PJI，即使需要调整银的含量以提高骨整合。
