Bohdan Khmelnitsky National University, Shevchenka Str. 81, Cherkassy 18031, Ukraine.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 May;108:115-22. doi: 10.1016/j.saa.2013.01.049. Epub 2013 Feb 6.
In this present study, silver nanoparticles were synthesized using synthetic humic substances (HSs) as reducing and stabilizing agents. Preference of synthetic HSs over natural humic matter is determined by a standardization problem resolution of the product due to the strict control of conditions of the synthetic HSs formation. It allows to receive the silver nanoparticles with the standardized biologically-active protective shell that is very important for their use, mainly in medicine. The concentration of sodium hydroxide, synthetic HSs, silver nitrate and temperature employed in the synthesis process are optimized to attain better yield, controlled size and stability by means of UV-visible technique. In the optimal reaction conditions the concentrated silver colloids (55 mM) with 99.99% yield are obtained which were stable for more than 1 year under ambient conditions. The received silver nanoparticles are characterized by UV-visible spectroscopy, X-ray diffraction (XRD), FT-IR spectroscopy and transmission electron microscopy (TEM). The antimicrobial activity of silver nanoparticles against fungal and bacterial strains is also shown.
在本研究中，使用合成腐殖质（HSs）作为还原剂和稳定剂来合成银纳米粒子。与天然腐殖质相比，合成 HSs 的优势在于通过标准化问题解决了产品的问题，因为合成 HSs 形成的条件受到严格控制。这使得可以获得具有标准化生物活性保护壳的银纳米粒子，这对于它们的使用非常重要，主要是在医学领域。通过使用 UV-可见技术优化了合成过程中氢氧化钠、合成 HSs、硝酸银和温度的浓度，以获得更好的产率、可控的尺寸和稳定性。在最佳反应条件下，获得了产率为 99.99%的浓度为 55mM 的浓缩银胶体，在环境条件下稳定超过 1 年。通过紫外可见光谱、X 射线衍射（XRD）、傅里叶变换红外光谱和透射电子显微镜（TEM）对所得到的银纳米粒子进行了表征。还展示了银纳米粒子对真菌和细菌菌株的抗菌活性。
