Subramanyam Gunashekar Kalvakunta, Gaddam Susmila Aparna, Kotakadi Venkata Subbaiah, Gunti Hema, Palithya Sashikiran, Penchalaneni Josthna, Challagundla Varadarajulu Naidu
Department of Biotechnology, Dravidian University, Kuppam, Andhra Pradesh, India.
Department of Virology, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):83-94. doi: 10.1080/21691401.2023.2173218.
The present research was carried out to look into therapeutic insight of biosynthesized silver nanoparticles (AgNPs) by leaf extract of Roxb (BH). The analysis of biosynthesized BH-AgNPs by UV-visible spectroscopy shows an intense surface plasmon resonance (SPR) peak at 422 nm initially and 437 nm after 30 min which certainly reveals the formation of BH-AgNPs. Fourier Infra-red Spectroscopy (FT-IR) reveals that BH-AgNPs are biosynthesized by using different bioactive compounds like O-H stretch of free hydroxyl alcohol and phenols, N-H bond of primary amines present in the leaf extract. Transmission Electron Microscope (TEM) analysis revealed that BH-AgNPs are almost spherical in nature with an average size range from of 2 nm to 12 nm. The particle size analysis by Dynamic Light Scattering (DLS) reveals that the BH-AgNPs are poly-dispersed in nature with an average size of 8 nm ± 2 nm, with a negative zeta potential value of -21 mV which reveals the biosynthesized BH-AgNPs are very stable. The BH-AgNPs -AgNPs revealed excellent free radical scavenging activity and exceptional antimicrobial activity. The anti-proliferative and cytotoxic studies in KB oral cancer cells revealed biosynthesized BH-AgNPs can employ as future novel therapeutic agents in cancer treatment and other biomedical applications.
本研究旨在探究由罗克斯伯（BH）叶提取物生物合成的银纳米颗粒（AgNPs）的治疗前景。通过紫外可见光谱对生物合成的BH-AgNPs进行分析，结果显示最初在422nm处有一个强烈的表面等离子体共振（SPR）峰，30分钟后在437nm处出现该峰，这无疑表明了BH-AgNPs的形成。傅里叶变换红外光谱（FT-IR）表明，BH-AgNPs是通过使用不同的生物活性化合物生物合成的，如游离羟基醇和酚的O-H伸缩振动、叶提取物中存在的伯胺的N-H键。透射电子显微镜（TEM）分析表明，BH-AgNPs本质上几乎呈球形，平均尺寸范围为2nm至12nm。通过动态光散射（DLS）进行的粒度分析表明，BH-AgNPs本质上是多分散的，平均尺寸为8nm±2nm，zeta电位值为-21mV，呈负值，这表明生物合成的BH-AgNPs非常稳定。BH-AgNPs显示出优异的自由基清除活性和出色的抗菌活性。在KB口腔癌细胞中的抗增殖和细胞毒性研究表明，生物合成的BH-AgNPs可作为未来癌症治疗和其他生物医学应用中的新型治疗剂。
