没食子儿茶素没食子酸酯促进猪卵母细胞体外受精后体外成熟和胚胎发育。
Epigallocatechin-3-Gallate Promotes the in vitro Maturation and Embryo Development Following IVF of Porcine Oocytes.
机构信息
National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Department of Tropical Agriculture and Forestry, College of Guangdong Agriculture Industry Business Polytechnic, Guangzhou, Guangdong, 510507, People's Republic of China.
出版信息
Drug Des Devel Ther. 2021 Mar 4;15:1013-1020. doi: 10.2147/DDDT.S295936. eCollection 2021.
PURPOSE
Epigallocatechin-3-gallate (EGCG) is a major ingredient of catechin polyphenols and exerts protective effects because of its strong antioxidant properties. As far as we know, there is still a lack of systematic research on the effects of EGCG on the in vitro maturation (IVM) and in vitro fertilization (IVF) of porcine oocytes. The present study aimed to determine the effects of EGCG on the IVM and IVF of porcine oocytes.
METHODS
Porcine oocytes were treated with different concentrations of EGCG (5, 10 and 20 µM), and the cumulus cell expansion, oocyte maturation rate, reactive oxygen species (ROS), glutathione (GSH) and malondialdehyde (MDA) levels, total antioxidant capacity were determined. The mRNA expression levels of oxidative stress- and apoptosis-associated genes were determined by quantitative real-time PCR. The cleavage rate and blastocyst rate of oocytes after 10 μM EGCG treatment during IVM and IVF were also evaluated.
RESULTS
EGCG at 5, 10 and 20 μM significantly promoted cumulus cell expansion, and EGCG at 10 μM increased the oocyte maturation rate. EGCG (10 μM) treatment reduced the ROS and MDA levels, while increased the antioxidant capacity and GSH concentrations in the mature oocytes. The qRT-PCR results showed that EGCG treatment up-regulated the mRNA expression of catalase, glutathione peroxidase and superoxide dismutase in the mature oocytes. In addition, EGCG treatment also decreased the mRNA expression levels of Bax and caspase-3 and increased the Bcl-2 mRNA expression level in the mature oocytes. In addition, the cleavage rate and blastocyst rate of oocytes treated with 10 μM EGCG during IVM and IVF were significantly higher than those of the control group.
CONCLUSION
Our results suggest that EGCG promotes the in vitro maturation and embryo development following IVF of porcine oocytes. The protective effects of EGCG on the oocytes may be associated with its antioxidant and anti-apoptosis properties.
目的
表没食子儿茶素没食子酸酯（EGCG）是儿茶素多酚的主要成分，因其具有很强的抗氧化特性而发挥保护作用。据我们所知，目前仍然缺乏关于 EGCG 对猪卵母细胞体外成熟（IVM）和体外受精（IVF）影响的系统研究。本研究旨在确定 EGCG 对猪卵母细胞 IVM 和 IVF 的影响。
方法
用不同浓度的 EGCG（5、10 和 20 μM）处理猪卵母细胞，测定卵丘细胞扩展、卵母细胞成熟率、活性氧（ROS）、谷胱甘肽（GSH）和丙二醛（MDA）水平、总抗氧化能力。通过定量实时 PCR 测定氧化应激和凋亡相关基因的 mRNA 表达水平。还评估了 10 μM EGCG 在 IVM 和 IVF 期间处理卵母细胞对卵母细胞的卵裂率和囊胚率的影响。
结果
5、10 和 20 μM 的 EGCG 显著促进了卵丘细胞的扩展，而 10 μM 的 EGCG 提高了卵母细胞的成熟率。EGCG（10 μM）处理降低了 ROS 和 MDA 水平，同时增加了成熟卵母细胞中的抗氧化能力和 GSH 浓度。qRT-PCR 结果显示，EGCG 处理上调了成熟卵母细胞中过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶的 mRNA 表达。此外，EGCG 处理还降低了 Bax 和 caspase-3 的 mRNA 表达水平，增加了成熟卵母细胞中 Bcl-2 的 mRNA 表达水平。此外，IVM 和 IVF 期间用 10 μM EGCG 处理的卵母细胞的卵裂率和囊胚率明显高于对照组。
结论
我们的结果表明，EGCG 促进了猪卵母细胞的体外成熟和随后的 IVF 胚胎发育。EGCG 对卵母细胞的保护作用可能与其抗氧化和抗凋亡特性有关。
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