针对曲霉属真菌对棘白菌素类药物的药敏试验，Sensititre YeastOne方法与CLSI M38 - A2参考方法的比较评估
Comparative Evaluation of Sensititre YeastOne and CLSI M38-A2 Reference Method for Antifungal Susceptibility Testing of Aspergillus spp. against Echinocandins.
作者信息
Siopi Maria, Pournaras Spyros, Meletiadis Joseph
机构信息
Clinical Microbiology Laboratory, Attikon University General Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Clinical Microbiology Laboratory, Attikon University General Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece
出版信息
J Clin Microbiol. 2017 Jun;55(6):1714-1719. doi: 10.1128/JCM.00044-17. Epub 2017 Mar 22.
Abstract
Sensititre YeastOne (YO) panels were assessed for susceptibility testing of echinocandins against 39 isolates of , , and , including two echinocandin-resistant strains, using different inocula (10, 10, and 10 CFU/ml), incubation times (16 to 48 h), and endpoints (first blue or purple well) and compared to CLSI M38-A2. The best agreement was found with an inoculum of 10 CFU/ml, incubation times of 20 h for and of 30 h for and , and reading the first purple well. The reproducibility within ±1 2-fold dilutions was 100% for all three echinocandins. YO color endpoints were 2 to 3 2-fold dilutions lower than CLSI minimum effective concentrations (MECs) of caspofungin and 1 to 2 2-fold dilutions higher than CLSI MECs of micafungin. For anidulafungin, off-scale YO color endpoints were observed. Nevertheless, echinocandin-resistant isolates were detected after 24 h of incubation.
摘要
使用不同接种量（10、10和10 CFU/ml）、孵育时间（16至48小时）和终点（第一个蓝色或紫色孔），对Sensititre YeastOne（YO）平板进行了棘白菌素对39株、和的敏感性测试，其中包括两株棘白菌素耐药菌株，并与CLSI M38 - A2进行比较。发现接种量为10 CFU/ml、对孵育20小时、对和孵育30小时并读取第一个紫色孔时一致性最佳。所有三种棘白菌素在±1 2倍稀释范围内的重现性均为100%。YO颜色终点比卡泊芬净的CLSI最低有效浓度（MEC）低2至3个2倍稀释度，比米卡芬净的CLSI MEC高1至2个2倍稀释度。对于阿尼芬净，观察到超出范围的YO颜色终点。然而，孵育24小时后检测到了棘白菌素耐药菌株。
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