Warfel Jaycob D, Vandanmagsar Bolormaa, Dubuisson Olga S, Hodgeson Sydney M, Elks Carrie M, Ravussin Eric, Mynatt Randall L
Gene Nutrient Interactions Laboratory, Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, Louisiana.
Matrix Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, Louisiana; and.
Am J Physiol Regul Integr Comp Physiol. 2017 May 1;312(5):R816-R820. doi: 10.1152/ajpregu.00520.2016. Epub 2017 Mar 22.
Carnitine palmitoyltransferase 1 (CPT1) is essential for the transport of long-chain fatty acids into the mitochondria for oxidation. Recently, it was reported that decreased CPT1b mRNA in adipose tissue was a contributing factor for obesity in rats. We therefore closely examined the expression level of in adipose tissue from mice, rats, and humans. is the predominate isoform in adipose tissue from all three species. Rat white adipose tissue has a moderate amount of mRNA, but it is very minor compared with expression in muscle. Total CPT1 activity in adipose tissue is also minor relative to other tissues. Both and mRNA were increased in gonadal fat but not inguinal fat by diet-induced obesity in mice. We also measured and expression in subcutaneous adipose tissue from human subjects with a wide range of body mass indexes (BMIs). Interestingly, expression positively correlated with BMI ( = 0.46), but there was no correlation with ( = 0.04). Our findings indicate that white adipose tissue fatty acid oxidation capacity is minor compared with that of metabolically active tissues. Furthermore, given the already low abundance of in white adipose tissue, it is unlikely that decreases in its expression can quantitatively decrease whole body energy expenditure enough to contribute to an obese phenotype.
肉碱棕榈酰转移酶1（CPT1）对于长链脂肪酸转运至线粒体进行氧化至关重要。最近有报道称，大鼠脂肪组织中CPT1b mRNA水平降低是导致肥胖的一个因素。因此，我们仔细检测了小鼠、大鼠和人类脂肪组织中的表达水平。是这三个物种脂肪组织中的主要异构体。大鼠白色脂肪组织中有适量的mRNA，但与肌肉中的表达相比非常少。脂肪组织中的总CPT1活性相对于其他组织也很低。饮食诱导的小鼠肥胖使性腺脂肪中的和mRNA均增加，但腹股沟脂肪中未增加。我们还检测了一系列体重指数（BMI）的人类受试者皮下脂肪组织中的和表达。有趣的是，表达与BMI呈正相关（= 0.46），但与无相关性（= 0.04）。我们的研究结果表明，白色脂肪组织的脂肪酸氧化能力与代谢活跃组织相比很低。此外，鉴于白色脂肪组织中本就含量低，其表达降低不太可能在数量上使全身能量消耗减少到足以导致肥胖表型的程度。
