一种以1,N6-乙烯基腺苷酸为底物的5'-核苷酸酶新检测方法，以及对反应产物乙烯基腺苷特性的评论。
A novel assay for 5'-nucleotidase using 1,N6-etheno-AMP as substrate, and comments on the properties of the reaction product, ethenoadenosine.
作者信息
Jamal Z, Afkham-Ebrahimi A, Saggerson E D
机构信息
Department of Biochemistry, University College London, U.K.
出版信息
Biochem J. 1988 Mar 1;250(2):369-73. doi: 10.1042/bj2500369.
Abstract
A novel assay for 5'-nucleotidase is described in which 1,N6-etheno-AMP is converted into ethenoadenosine. The product ethenoadenosine is neither a substrate for nor an inhibitor of adenosine deaminase. Ethenoadenosine appears to have little effect at adenosine receptors on adipose-tissue cells.
摘要
本文描述了一种用于5'-核苷酸酶的新型检测方法，其中1,N6-乙烯基腺苷酸被转化为乙烯基腺苷。产物乙烯基腺苷既不是腺苷脱氨酶的底物，也不是其抑制剂。乙烯基腺苷似乎对脂肪组织细胞上的腺苷受体影响很小。
相似文献
1
A novel assay for 5'-nucleotidase using 1,N6-etheno-AMP as substrate, and comments on the properties of the reaction product, ethenoadenosine.一种以1,N6-乙烯基腺苷酸为底物的5'-核苷酸酶新检测方法，以及对反应产物乙烯基腺苷特性的评论。
Biochem J. 1988 Mar 1;250(2):369-73. doi: 10.1042/bj2500369.
2
Adenosine and the control of lipolysis in rat adipocytes during pregnancy and lactation.孕期和哺乳期大鼠脂肪细胞中腺苷与脂肪分解的调控
Biochem J. 1983 Oct 15;216(1):121-8. doi: 10.1042/bj2160121.
3
Activities of adenylate-degrading enzymes in muscles from vertebrates and invertebrates.脊椎动物和无脊椎动物肌肉中腺苷酸降解酶的活性。
Comp Biochem Physiol B. 1987;86(1):109-12. doi: 10.1016/0305-0491(87)90183-0.
4
Distribution of adenosine metabolising enzymes between adipocyte and stromal-vascular cells of adipose tissue.脂肪组织中脂肪细胞与基质血管细胞之间腺苷代谢酶的分布。
Biochim Biophys Acta. 1981 Aug 5;676(1):122-4. doi: 10.1016/0304-4165(81)90017-9.
5
Enzymes involved in adenosine metabolism in rat white and brown adipocytes. Effects of streptozotocin-diabetes, hypothyroidism, age and sex differences.大鼠白色和棕色脂肪细胞中参与腺苷代谢的酶。链脲佐菌素诱导的糖尿病、甲状腺功能减退、年龄和性别差异的影响。
Biochem J. 1987 Aug 1;245(3):881-6. doi: 10.1042/bj2450881.
6
AMP phosphatase activity in human term placenta: studies on placental 5'-nucleotidase.人足月胎盘的AMP磷酸酶活性：关于胎盘5'-核苷酸酶的研究
Adv Exp Med Biol. 1980;122A:393-5. doi: 10.1007/978-1-4615-9140-5_63.
7
Activities and some properties of 5'-nucleotidase, adenosine kinase and adenosine deaminase in tissues from vertebrates and invertebrates in relation to the control of the concentration and the physiological role of adenosine.脊椎动物和无脊椎动物组织中5'-核苷酸酶、腺苷激酶和腺苷脱氨酶的活性及某些特性与腺苷浓度调控和生理作用的关系
Biochem J. 1978 Sep 15;174(3):965-77. doi: 10.1042/bj1740965.
8
The proposed 5'-nucleotidase inhibitor in human leukemic cells is an artefact.在人类白血病细胞中所提出的5'-核苷酸酶抑制剂是一种假象。
Biochim Biophys Acta. 1984 Aug 17;804(4):398-403. doi: 10.1016/0167-4889(84)90066-1.
9
AMP deaminase, 5'-nucleotidase and adenosine deaminase in rat myocardial tissue in myocardial infarction and hypothermia.心肌梗死和低温状态下大鼠心肌组织中的AMP脱氨酶、5'-核苷酸酶和腺苷脱氨酶
Experientia. 1982 Jul 15;38(7):776-7. doi: 10.1007/BF01972265.
10
Maximum activities of enzymes involved in adenosine metabolism in adipose tissue of rats and mice under conditions of variations in insulin sensitivity.在胰岛素敏感性发生变化的情况下，大鼠和小鼠脂肪组织中参与腺苷代谢的酶的最大活性。
Biochim Biophys Acta. 1981 Aug 5;676(1):125-8. doi: 10.1016/0304-4165(81)90018-0.
引用本文的文献
1
Nanomolar clodronate induces adenosine accumulation in the perfused rat mesenteric bed and mesentery-derived endothelial cells.纳摩尔浓度的氯膦酸盐可诱导灌注大鼠肠系膜床和肠系膜来源的内皮细胞中腺苷的积累。
Front Pharmacol. 2023 Jan 20;13:1031223. doi: 10.3389/fphar.2022.1031223. eCollection 2022.
2
Identification of the Mouse T Cell ADP-Ribosylome Uncovers ARTC2.2 Mediated Regulation of CD73 by ADP-Ribosylation.鉴定小鼠 T 细胞 ADP-核糖基组揭示了 ARTC2.2 通过 ADP-核糖基化对 CD73 的调控作用。
Front Immunol. 2021 Aug 24;12:703719. doi: 10.3389/fimmu.2021.703719. eCollection 2021.
3
Extracellular metabolism of the enteric inhibitory neurotransmitter β-nicotinamide adenine dinucleotide (β-NAD) in the murine colon.肠道抑制性神经递质 β-烟酰胺腺嘌呤二核苷酸（β-NAD）在鼠结肠中的细胞外代谢。
J Physiol. 2020 Oct;598(20):4509-4521. doi: 10.1113/JP280051. Epub 2020 Aug 13.
4
An ex vivo bladder model with detrusor smooth muscle removed to analyse biologically active mediators released from the suburothelium.一种去除逼尿肌平滑肌的离体膀胱模型，用于分析从膀胱下黏膜释放的具有生物活性的介质。
J Physiol. 2019 Mar;597(6):1467-1485. doi: 10.1113/JP276924. Epub 2018 Nov 2.
5
Up-regulated Ectonucleotidases in Fas-Associated Death Domain Protein- and Receptor-Interacting Protein Kinase 1-Deficient Jurkat Leukemia Cells Counteract Extracellular ATP/AMP Accumulation via Pannexin-1 Channels during Chemotherapeutic Drug-Induced Apoptosis.在化疗药物诱导的凋亡过程中，Fas相关死亡结构域蛋白和受体相互作用蛋白激酶1缺陷的Jurkat白血病细胞中上调的外核苷酸酶通过泛连接蛋白1通道抵消细胞外ATP/AMP的积累。
Mol Pharmacol. 2017 Jul;92(1):30-47. doi: 10.1124/mol.116.104000. Epub 2017 May 1.
6
A commonly used ecto-ATPase inhibitor, ARL-67156, blocks degradation of ADP more than the degradation of ATP in murine colon.一种常用的胞外ATP酶抑制剂ARL-67156，在小鼠结肠中对ADP降解的阻断作用比对ATP降解的阻断作用更强。
Neurogastroenterol Motil. 2016 Sep;28(9):1370-81. doi: 10.1111/nmo.12836. Epub 2016 Apr 5.
7
Adenosine 5-diphosphate-ribose is a neural regulator in primate and murine large intestine along with β-NAD(+).三磷酸腺苷二核糖是灵长类动物和鼠类大肠中的一种神经调节剂，与 β-NAD(+) 一起。
J Physiol. 2012 Apr 15;590(8):1921-41. doi: 10.1113/jphysiol.2011.222414. Epub 2012 Feb 20.
8
Release, neuronal effects and removal of extracellular β-nicotinamide adenine dinucleotide (β-NAD⁺) in the rat brain.β-烟酰胺腺嘌呤二核苷酸（β-NAD⁺）在大鼠脑内的释放、神经元作用和清除。
Eur J Neurosci. 2012 Feb;35(3):423-35. doi: 10.1111/j.1460-9568.2011.07957.x. Epub 2012 Jan 25.
9
Ecto-5'-nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia.缺氧诱导因子-1对ecto-5'-核苷酸酶（CD73）的调控介导肠道上皮细胞的通透性变化。
J Clin Invest. 2002 Oct;110(7):993-1002. doi: 10.1172/JCI15337.
10
Differences in the properties of A1-type adenosine receptors in rat white and brown adipocytes.大鼠白色和棕色脂肪细胞中A1型腺苷受体特性的差异。
Biochem J. 1990 Jul 1;269(1):157-61. doi: 10.1042/bj2690157.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.分离脂肪细胞的代谢。I. 激素对葡萄糖代谢和脂肪分解的影响。
J Biol Chem. 1964 Feb;239:375-80.
3
Adenosine diphosphate in red cells as a factor in the adhesiveness of human blood platelets.红细胞中的二磷酸腺苷作为人类血小板黏附性的一个因素。
Nature. 1961 Nov 11;192:531-2. doi: 10.1038/192531a0.
4
The brown fat cell.棕色脂肪细胞。
Int Rev Cytol. 1982;74:187-286. doi: 10.1016/s0074-7696(08)61173-0.
5
A proposal for the role of ecto-enzymes and adenylates in traumatic shock.关于外切酶和腺苷酸在创伤性休克中作用的一项提议。
J Theor Biol. 1980 Dec 7;87(3):609-21. doi: 10.1016/0022-5193(80)90239-8.
6
Adenosine and the control of lipolysis in rat adipocytes during pregnancy and lactation.孕期和哺乳期大鼠脂肪细胞中腺苷与脂肪分解的调控
Biochem J. 1983 Oct 15;216(1):121-8. doi: 10.1042/bj2160121.
7
Inhibition of pinocytosis in rat embryo fibroblasts treated with monensin.莫能菌素处理的大鼠胚胎成纤维细胞中胞饮作用的抑制
J Cell Biol. 1982 Mar;92(3):859-64. doi: 10.1083/jcb.92.3.859.
8
Evidence for a continual exchange of 5'-nucleotidase between the cell surface and cytoplasmic membranes in cultured rat fibroblasts.培养的大鼠成纤维细胞中5'-核苷酸酶在细胞表面和细胞质膜之间持续交换的证据。
Cell. 1982 Jan;28(1):61-70. doi: 10.1016/0092-8674(82)90375-0.
9
Maximum activities of enzymes involved in adenosine metabolism in adipose tissue of rats and mice under conditions of variations in insulin sensitivity.在胰岛素敏感性发生变化的情况下，大鼠和小鼠脂肪组织中参与腺苷代谢的酶的最大活性。
Biochim Biophys Acta. 1981 Aug 5;676(1):125-8. doi: 10.1016/0304-4165(81)90018-0.
10
Subcellular distribution and movement of 5'-nucleotidase in rat cells.大鼠细胞中5'-核苷酸酶的亚细胞分布与移动
Biochem J. 1980 Jan 15;186(1):59-69. doi: 10.1042/bj1860059.
Zotero 插件