一种以1,N6-乙烯基腺苷酸为底物的5'-核苷酸酶新检测方法，以及对反应产物乙烯基腺苷特性的评论。
A novel assay for 5'-nucleotidase using 1,N6-etheno-AMP as substrate, and comments on the properties of the reaction product, ethenoadenosine.
作者信息
Jamal Z, Afkham-Ebrahimi A, Saggerson E D
机构信息
Department of Biochemistry, University College London, U.K.
出版信息
Biochem J. 1988 Mar 1;250(2):369-73. doi: 10.1042/bj2500369.
Abstract
A novel assay for 5'-nucleotidase is described in which 1,N6-etheno-AMP is converted into ethenoadenosine. The product ethenoadenosine is neither a substrate for nor an inhibitor of adenosine deaminase. Ethenoadenosine appears to have little effect at adenosine receptors on adipose-tissue cells.
摘要
本文描述了一种用于5'-核苷酸酶的新型检测方法，其中1,N6-乙烯基腺苷酸被转化为乙烯基腺苷。产物乙烯基腺苷既不是腺苷脱氨酶的底物，也不是其抑制剂。乙烯基腺苷似乎对脂肪组织细胞上的腺苷受体影响很小。
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