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一种以1,N6-乙烯基腺苷酸为底物的5'-核苷酸酶新检测方法，以及对反应产物乙烯基腺苷特性的评论。

A novel assay for 5'-nucleotidase using 1,N6-etheno-AMP as substrate, and comments on the properties of the reaction product, ethenoadenosine.

作者信息

Jamal Z, Afkham-Ebrahimi A, Saggerson E D

机构信息

Department of Biochemistry, University College London, U.K.

出版信息

Biochem J. 1988 Mar 1;250(2):369-73. doi: 10.1042/bj2500369.

DOI:10.1042/bj2500369
PMID:2833240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1148865/
Abstract

A novel assay for 5'-nucleotidase is described in which 1,N6-etheno-AMP is converted into ethenoadenosine. The product ethenoadenosine is neither a substrate for nor an inhibitor of adenosine deaminase. Ethenoadenosine appears to have little effect at adenosine receptors on adipose-tissue cells.

摘要

本文描述了一种用于5'-核苷酸酶的新型检测方法，其中1,N6-乙烯基腺苷酸被转化为乙烯基腺苷。产物乙烯基腺苷既不是腺苷脱氨酶的底物，也不是其抑制剂。乙烯基腺苷似乎对脂肪组织细胞上的腺苷受体影响很小。

相似文献

1
A novel assay for 5'-nucleotidase using 1,N6-etheno-AMP as substrate, and comments on the properties of the reaction product, ethenoadenosine.一种以1,N6-乙烯基腺苷酸为底物的5'-核苷酸酶新检测方法，以及对反应产物乙烯基腺苷特性的评论。
Biochem J. 1988 Mar 1;250(2):369-73. doi: 10.1042/bj2500369.
2
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3
Activities of adenylate-degrading enzymes in muscles from vertebrates and invertebrates.脊椎动物和无脊椎动物肌肉中腺苷酸降解酶的活性。
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7
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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.分离脂肪细胞的代谢。I. 激素对葡萄糖代谢和脂肪分解的影响。
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Adenosine and the control of lipolysis in rat adipocytes during pregnancy and lactation.孕期和哺乳期大鼠脂肪细胞中腺苷与脂肪分解的调控
Biochem J. 1983 Oct 15;216(1):121-8. doi: 10.1042/bj2160121.
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Inhibition of pinocytosis in rat embryo fibroblasts treated with monensin.莫能菌素处理的大鼠胚胎成纤维细胞中胞饮作用的抑制
J Cell Biol. 1982 Mar;92(3):859-64. doi: 10.1083/jcb.92.3.859.
8
Evidence for a continual exchange of 5'-nucleotidase between the cell surface and cytoplasmic membranes in cultured rat fibroblasts.培养的大鼠成纤维细胞中5'-核苷酸酶在细胞表面和细胞质膜之间持续交换的证据。
Cell. 1982 Jan;28(1):61-70. doi: 10.1016/0092-8674(82)90375-0.
9
Maximum activities of enzymes involved in adenosine metabolism in adipose tissue of rats and mice under conditions of variations in insulin sensitivity.在胰岛素敏感性发生变化的情况下，大鼠和小鼠脂肪组织中参与腺苷代谢的酶的最大活性。
Biochim Biophys Acta. 1981 Aug 5;676(1):125-8. doi: 10.1016/0304-4165(81)90018-0.
10
Subcellular distribution and movement of 5'-nucleotidase in rat cells.大鼠细胞中5'-核苷酸酶的亚细胞分布与移动
Biochem J. 1980 Jan 15;186(1):59-69. doi: 10.1042/bj1860059.