Li Fen, Ma Kang-Sheng, Liang Ping-Zhuo, Chen Xue-Wei, Liu Ying, Gao Xi-Wu
Department of Entomology China Agricultural University, Beijing 100193, China (
J Econ Entomol. 2017 Apr 1;110(2):624-631. doi: 10.1093/jee/tow322.
Aphis gossypii Glover (Hemiptera: Aphididae) can damage a variety of agricultural crops, so it is very important for cotton aphids to evolve adaptive mechanisms to various allelochemicals from host plants. Our results aim to provide a fundamental and rich resource for exploring aphid functional genes in A. gossypii. A transcriptome data set and five expression profile data sets of A. gossypii samples were analyzed by Illumina sequencing platform. In total, 53,763,866 reads were assembled into 1,963,516 contigs and 28,555 unigenes. Compared with the control, 619 genes were significantly up- or downregulated in the treatment group by 2-tridecanone. There were 516, 509, and 717 of differential expression genes in tannic acid, quercetin, and gossypol treatment groups, respectively. Furthermore, there were 4 of 54 putative cytochrome P450 genes and 1 of 7 putative carboxylesterases downregulated in all treatment groups by four plant allelochemicals. When aphids fed on 2-tridecanone, tannic acid, and quercetin, only one P450 gene was upregulated. These results show that plant allelochemical stress can induce differential gene expression in A. gossypii. The differential response information of gene expression based on a large-scale sequence would be useful to reveal molecular mechanisms of adaptation for A. gossypii to plant allelochemicals.
棉蚜（半翅目：蚜科）会损害多种农作物，因此棉蚜进化出针对寄主植物各种化感物质的适应机制非常重要。我们的研究结果旨在为探索棉蚜中的蚜虫功能基因提供基础且丰富的资源。利用Illumina测序平台分析了棉蚜样本的转录组数据集和五个表达谱数据集。总共53,763,866条 reads 被组装成1,963,516个重叠群和28,555个单基因。与对照组相比，在2-十三烷酮处理组中有619个基因显著上调或下调。单宁酸、槲皮素和棉酚处理组中分别有516、509和717个差异表达基因。此外，在所有处理组中，54个假定的细胞色素P450基因中有4个以及7个假定的羧酸酯酶中有1个被四种植物化感物质下调。当蚜虫取食2-十三烷酮、单宁酸和槲皮素时，只有一个P450基因上调。这些结果表明植物化感物质胁迫可诱导棉蚜基因差异表达。基于大规模序列的基因表达差异响应信息将有助于揭示棉蚜对植物化感物质的适应分子机制。
