State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China ; Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
PLoS One. 2013 Dec 23;8(12):e83180. doi: 10.1371/journal.pone.0083180. eCollection 2013.
The cotton aphid, Aphis gossypii Glover, is a destructive insect pest worldwide; it directly or indirectly damages (virus transmission) 300 species of host plants. Knowledge of their ecologically adaptive mechanisms at the molecular level may provide an essential and urgent method to effectively control this pest. However, no transcriptome information is available for the cotton aphid and sequence data are scarce. Therefore, we obtained transcriptome data.
To facilitate such a study, two cotton aphid transcriptomes at different growth stages of cotton, seedling and summer, were sequenced. A total of 161,396 and 66,668 contigs were obtained and assembled into 83,671 and 42,438 transcripts, respectively. After combining the raw date for both transcriptomes, the sequences were reassembled into 66,695 transcripts, and 52,160 were annotated based on BLASTX analyses. Comparison of the transcriptomes revealed that summer presented less challenges for the cotton aphids than the seedling stage of cotton. In total, 58 putative heat shock protein genes and 66 candidate cytochrome p450 genes were identified with BLASTX.
Our results form a basis for exploring the molecular mechanisms of ecological adaption in the cotton aphid. Our study also provides a baseline for the exploration of abiotic stress responses. In addition, it provides large-scale sequence information for further studies on this species.
棉蚜，Aphis gossypii Glover，是一种具有全球破坏性的昆虫害虫；它直接或间接损害（病毒传播）300 种宿主植物。在分子水平上了解其生态适应性机制可能是有效控制这种害虫的必要和紧迫方法。然而，棉蚜没有转录组信息，序列数据也很稀缺。因此，我们获得了转录组数据。
为了便于进行这样的研究，我们对棉花幼苗期和夏季两个不同生长阶段的棉蚜进行了转录组测序。共获得 161396 个和 66668 个 contigs，并分别组装成 83671 个和 42438 个转录本。将两个转录组的原始数据合并后，将序列重新组装成 66695 个转录本，根据 BLASTX 分析，有 52160 个被注释。对转录组的比较表明，夏季对棉蚜的挑战小于棉花幼苗期。总共鉴定出 58 个推定热休克蛋白基因和 66 个候选细胞色素 P450 基因。
我们的结果为探索棉蚜生态适应的分子机制奠定了基础。我们的研究还为探索非生物胁迫反应提供了基线。此外，它为进一步研究该物种提供了大规模的序列信息。
