De Sousa Paul A, Steeg Rachel, Wachter Elisabeth, Bruce Kevin, King Jason, Hoeve Marieke, Khadun Shalinee, McConnachie George, Holder Julie, Kurtz Andreas, Seltmann Stefanie, Dewender Johannes, Reimann Sascha, Stacey Glyn, O'Shea Orla, Chapman Charlotte, Healy Lyn, Zimmermann Heiko, Bolton Bryan, Rawat Trisha, Atkin Isobel, Veiga Anna, Kuebler Bernd, Serano Blanca Miranda, Saric Tomo, Hescheler Jürgen, Brüstle Oliver, Peitz Michael, Thiele Cornelia, Geijsen Niels, Holst Bjørn, Clausen Christian, Lako Majlinda, Armstrong Lyle, Gupta Shailesh K, Kvist Alexander J, Hicks Ryan, Jonebring Anna, Brolén Gabriella, Ebneth Andreas, Cabrera-Socorro Alfredo, Foerch Patrik, Geraerts Martine, Stummann Tina C, Harmon Shawn, George Carol, Streeter Ian, Clarke Laura, Parkinson Helen, Harrison Peter W, Faulconbridge Adam, Cherubin Luca, Burdett Tony, Trigueros Cesar, Patel Minal J, Lucas Christa, Hardy Barry, Predan Rok, Dokler Joh, Brajnik Maja, Keminer Oliver, Pless Ole, Gribbon Philip, Claussen Carsten, Ringwald Annette, Kreisel Beate, Courtney Aidan, Allsopp Timothy E
Centre for Clinical Brain Sciences, Chancellors Building, 49 Little France Crescent, University of Edinburgh, Edinburgh EH16 4SB, UK; Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK.
Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK.
Stem Cell Res. 2017 Apr;20:105-114. doi: 10.1016/j.scr.2017.03.002. Epub 2017 Mar 7.
A fast track "Hot Start" process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement, bio-sample tracking, iPSC expansion, cryopreservation, qualification and distribution to the research community. These were implemented to create a quality managed foundational collection of lines and associated data made available for distribution. Here we report on the successful outcome of this experience and work flow for banking and facilitating access to an otherwise disparate European resource, with lessons to benefit the international research community. ETOC: The report focuses on the EBiSC experience of rapidly establishing an operational capacity to procure, bank and distribute a foundational collection of established hiPSC lines. It validates the feasibility and defines the challenges of harnessing and integrating the capability and productivity of centres across Europe using commonly available resources currently in the field.
实施了一个快速通道“热启动”流程来启动欧洲诱导多能干细胞银行（EBiSC），以便尽早发布一系列已建立的对照和与疾病相关的人类诱导多能干细胞（hiPSC）系。对联盟成员之间的既定做法进行了调查，以得出用于组织采购、生物样本追踪、iPSC扩增、冷冻保存、鉴定以及向研究界分发的统一且可公开获取的标准操作程序（SOP）。实施这些程序是为了创建一个经过质量管理的细胞系和相关数据基础库，以供分发。在此，我们报告这一经验和工作流程在银行存储以及促进获取原本分散的欧洲资源方面的成功成果，同时分享一些经验教训，以造福国际研究界。执行摘要：本报告重点介绍了EBiSC在迅速建立采购、存储和分发已建立的hiPSC系基础库的运营能力方面的经验。它验证了可行性，并确定了利用和整合欧洲各地中心的能力和生产力（使用该领域目前常用资源）所面临的挑战。
