Steeg Rachel, Neubauer Julia C, Müller Sabine C, Ebneth Andreas, Zimmermann Heiko
Fraunhofer UK Research Ltd, Technology and Innovation Centre, Glasgow G1 1RD, UK.
Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany.
Stem Cell Res. 2020 Dec;49:102034. doi: 10.1016/j.scr.2020.102034. Epub 2020 Oct 9.
The European Bank for induced Pluripotent Stem Cells (EBiSC), a non-profit repository for storage, banking, Quality Control (QC) and subsequent distribution of research-grade human induced Pluripotent Stem Cell (iPSC) lines, has centralised iPSC lines generated internationally across >35 disease areas and made them available to users via the EBiSC Catalogue, for research use (cells.ebisc.org/). Comprehensive datasets are accessible prior to purchase detailing the disease background of the original tissue sample, background of iPSC reprogramming and cell line characterisation data. EBiSC also performs robust QC screening to ensure supply of reliable, well-characterised iPSC lines, compliant with ISO9001:2015 principles. Whole Genome Sequencing data for specific iPSC lines can be downloaded from the European Genome Archive, subject to application to the EBiSC Data Access Committee. The EBiSC Access and Use Agreement, required to be completed prior to shipping, can be downloaded from the website along with specific Cell Line Information Packs; together these documents clarify how EBiSC lines can be used for research and detail any specific Third Party Obligations and/or restrictions for use which may apply. A protocol for how to culture and monitor iPSC lines including implementation of routine cell line screening is also available. A second project phase will continue collecting iPSC lines generated internationally, provide iPSC derived differentiated products using improved automation strategies for upscaling and develop the current services provided by EBiSC, including iPSC reprogramming, gene-editing and characterisation.
欧洲诱导多能干细胞库（EBiSC）是一个非营利性机构，用于储存、保存、质量控制（QC）以及随后分发研究级别的人类诱导多能干细胞（iPSC）系。它集中了国际上在超过35个疾病领域产生的iPSC系，并通过EBiSC目录将其提供给用户用于研究（cells.ebisc.org/）。在购买之前，可以获取全面的数据集，详细说明原始组织样本的疾病背景、iPSC重编程背景和细胞系特征数据。EBiSC还进行严格的质量控制筛选，以确保提供符合ISO9001:2015原则的可靠、特征明确的iPSC系。特定iPSC系的全基因组测序数据可从欧洲基因组档案馆下载，但需向EBiSC数据访问委员会提出申请。发货前需要完成的EBiSC访问和使用协议可从网站上下载，同时还有特定的细胞系信息包；这些文件一起阐明了EBiSC系如何用于研究，并详细说明了可能适用的任何特定第三方义务和/或使用限制。还提供了关于如何培养和监测iPSC系的方案，包括实施常规细胞系筛选。项目的第二阶段将继续收集国际上产生的iPSC系，使用改进的自动化策略扩大规模提供iPSC衍生的分化产品，并开发EBiSC目前提供的服务，包括iPSC重编程、基因编辑和特征分析。
