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缺乏泛素激活酶的小鼠温度敏感突变体ts85热耐受性发育缺陷。

Defect in the development of thermotolerance in the mouse temperature-sensitive mutant ts85 lacking ubiquitin-activating enzyme.

作者信息

Mizuno S, Ohkawara A, Suzuki K

机构信息

Department of Antibiotics, National Institute of Health, Tokyo.

出版信息

Jpn J Cancer Res. 1988 Jan;79(1):17-20. doi: 10.1111/j.1349-7006.1988.tb00005.x.

DOI:10.1111/j.1349-7006.1988.tb00005.x
PMID:2833480
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5907768/
Abstract

We investigated the effect of heat shock on the development of thermotolerance using mouse FM3A cells and the temperature-sensitive mutant ts85. The shift-up incubation of FM3A from 33 to 39.5 degrees induced thermotolerance to subsequent heating at 44 degrees. In contrast, the similar treatment of ts85 at the nonpermissive temperature of 39.5 degrees could not induce thermotolerance. Furthermore, when ts85 cells were treated at 33 degrees after being heated at 44 degrees, they developed a reduced level of thermotolerance as compared with that developed in FM3A cells. Since ts85 cells are defective in ubiquitin-activating enzyme, these results suggest a role of the ubiquitin-protein conjugation system in the development of thermotolerance.

摘要

我们使用小鼠FM3A细胞和温度敏感突变体ts85研究了热休克对耐热性发展的影响。将FM3A从33℃向上转移至39.5℃培养可诱导其对随后44℃加热产生耐热性。相比之下，在39.5℃的非允许温度下对ts85进行类似处理不能诱导耐热性。此外，当ts85细胞在44℃加热后于33℃处理时，与FM3A细胞相比，它们产生的耐热性水平降低。由于ts85细胞在泛素激活酶方面存在缺陷，这些结果表明泛素-蛋白质缀合系统在耐热性发展中起作用。

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本文引用的文献

1
The stress response: changes in eukaryotic gene expression in response to environmental stress.应激反应：真核生物基因表达随环境应激的变化。
Science. 1985 Nov 15;230(4727):800-1. doi: 10.1126/science.230.4727.800-a.
2
Relationship between hyperthermia-induced heat-shock proteins and thermotolerance in Morris hepatoma cells.高温诱导的热休克蛋白与莫里斯肝癌细胞耐热性之间的关系。
Can J Biochem Cell Biol. 1983 Jun;61(6):428-37. doi: 10.1139/o83-058.
3
Heat-stress proteins and thermal resistance in rat mammary tumor cells.大鼠乳腺肿瘤细胞中的热应激蛋白与热抗性
Radiat Res. 1983 Aug;95(2):399-413.
4
Dissociation of 68,000 Mr heat shock protein synthesis from thermotolerance expression in rat fibroblasts.大鼠成纤维细胞中68,000 Mr热休克蛋白合成与耐热性表达的解离。
Radiat Res. 1984 Aug;99(2):433-7.
5
The ubiquitin-mediated proteolytic pathway and mechanisms of energy-dependent intracellular protein degradation.泛素介导的蛋白水解途径及能量依赖性细胞内蛋白质降解机制。
J Cell Biochem. 1984;24(1):27-53. doi: 10.1002/jcb.240240104.
6
Ubiquitin dependence of selective protein degradation demonstrated in the mammalian cell cycle mutant ts85.在哺乳动物细胞周期突变体ts85中证实了选择性蛋白质降解对泛素的依赖性。
Cell. 1984 May;37(1):57-66. doi: 10.1016/0092-8674(84)90300-3.
7
Thermolability of ubiquitin-activating enzyme from the mammalian cell cycle mutant ts85.来自哺乳动物细胞周期突变体ts85的泛素激活酶的热稳定性
Cell. 1984 May;37(1):43-55. doi: 10.1016/0092-8674(84)90299-x.
8
Mechanisms of intracellular protein breakdown.细胞内蛋白质降解的机制。
Annu Rev Biochem. 1982;51:335-64. doi: 10.1146/annurev.bi.51.070182.002003.
9
Ubiquitin - protein conjugates.泛素-蛋白质缀合物
Mol Cell Biochem. 1981 Nov 13;40(3):173-87. doi: 10.1007/BF00224611.
10
Ethanol-induced cell sensitization to bleomycin cytotoxicity and the inhibition of recovery from potentially lethal damage.
Cancer Res. 1981 Oct;41(10):4111-4.

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