Landry J, Chrétien P
Can J Biochem Cell Biol. 1983 Jun;61(6):428-37. doi: 10.1139/o83-058.
We recently demonstrated that heat treatments, capable of triggering thermotolerance development in MH-7777 cells, also induce a transiently increased synthesis of a small set of polypeptides, a phenomenon similar to the heat-shock-protein (HSP) induction demonstrated in various organisms. A close temporal relationship was also found between HSP synthesis and thermotolerance development, and the degradation kinetics of the newly synthesized HSPs very closely paralleled the decay of the thermotolerant state. In the present study, we extended our analysis to determine whether various experimental conditions known to affect thermotolerance development (duration and temperature of the conditioning treatment, step-down heating, ethanol exposure) will also influence HSP synthesis and, conversely, whether agents known to induce (sodium arsenite) or to impair (cycloheximide) HSP production will similarly affect thermotolerance. The results suggested that the expression of HSP and thermotolerance in MH-7777 cells are most likely regulated by some interrelated mechanisms, but indicated that HSP synthesis is neither a sufficient or necessary condition for thermotolerance development.
我们最近证明，能够在MH-7777细胞中引发耐热性发展的热处理，也会诱导一小部分多肽的合成暂时增加，这一现象类似于在各种生物体中所证明的热休克蛋白（HSP）诱导。在HSP合成与耐热性发展之间也发现了紧密的时间关系，新合成的HSP的降解动力学与耐热状态的衰减非常相似。在本研究中，我们扩展了分析，以确定已知影响耐热性发展的各种实验条件（预处理的持续时间和温度、逐步降温加热、乙醇暴露）是否也会影响HSP合成，反之，已知诱导（亚砷酸钠）或损害（环己酰亚胺）HSP产生的试剂是否会同样影响耐热性。结果表明，MH-7777细胞中HSP的表达和耐热性很可能受一些相互关联的机制调节，但表明HSP合成既不是耐热性发展的充分条件也不是必要条件。
