Zu Yinxue, Bi Jingran, Yan Huiping, Wang Haitao, Song Yukun, Zhu Bei-Wei, Tan Mingqian
Liaoning Key Laboratory of Food Biological Technology, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan 1, Ganjingzi District, Dalian 116034, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Nanomaterials (Basel). 2016 Jul 5;6(7):130. doi: 10.3390/nano6070130.
Fluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Transmission electron microscopy (TEM) demonstrated that the average particle sizes are 14 nm and 75 nm for starch and chitosan NSs, respectively. Fourier transform infrared (FT-IR) spectroscopy analysis showed that there are a large number of hydroxyl or amino groups on the surface of these polysaccharide-based NSs. Strong fluorescence with an excitation-dependent emission behaviour was observed under ultraviolet excitation. Interestingly, the photostability of the NSs was found to be superior to fluorescein and rhodamine B. The quantum yield of starch NSs could reach 11.12% under the excitation of 360 nm. The oxidative metal ions including Cu(II), Hg(II)and Fe(III) exhibited a quench effect on the fluorescence intensity of the prepared NSs. Both of the two kinds of the multicoloured NSs showed a maximum fluorescence intensity at pH 7, while the fluorescence intensity decreased dramatically when they were put in an either acidic or basic environment (at pH 3 or 11). The cytotoxicity study of starch NSs showed that low cell cytotoxicity and 80% viability was found after 24 h incubation, when their concentration was less than 10 mg/mL. The study also showed the possibility of using the multicoloured starch NSs for mouse melanoma cells and guppy fish imaging.
源自多糖的荧光纳米结构（NSs）作为潜在生物成像应用的新型荧光探针备受关注。在此，我们报道了一种简便的碱辅助水热法，以淀粉和壳聚糖为原料制备多糖NSs。透射电子显微镜（TEM）表明，淀粉和壳聚糖NSs的平均粒径分别为14 nm和75 nm。傅里叶变换红外（FT-IR）光谱分析表明，这些基于多糖的NSs表面存在大量羟基或氨基。在紫外激发下观察到具有激发依赖发射行为的强荧光。有趣的是，发现NSs的光稳定性优于荧光素和罗丹明B。在360 nm激发下，淀粉NSs的量子产率可达11.12%。包括Cu(II)、Hg(II)和Fe(III)在内的氧化性金属离子对制备的NSs的荧光强度表现出淬灭作用。两种彩色NSs在pH 7时均表现出最大荧光强度，而当它们置于酸性或碱性环境（pH 3或11）中时，荧光强度急剧下降。淀粉NSs的细胞毒性研究表明，当浓度小于10 mg/mL时，孵育24 h后细胞毒性较低，存活率为80%。该研究还表明了使用彩色淀粉NSs对小鼠黑色素瘤细胞和孔雀鱼进行成像的可能性。
