壳聚糖和低聚壳聚糖包衣对姜黄素纳米混悬液黏膜黏附性的比较研究

Comparative Study of Chitosan and Oligochitosan Coatings on Mucoadhesion of Curcumin Nanosuspensions.

作者信息

Shin Gye Hwa, Kim Jun Tae

机构信息

Department of Food and Nutrition, Kunsan National University, Gunsan 54150, Korea.

Department of Food and Nutrition, BioNanocomposite Research Center, Kyung Hee University, Seoul 02447, Korea.

出版信息

Pharmaceutics. 2021 Dec 14;13(12):2154. doi: 10.3390/pharmaceutics13122154.

Curcumin nanosuspensions (Cur-NSs), chitosan-coated Cur-NSs (CS-Cur-NSs), and oligochitosan-coated Cur-NSs (OCS-Cur-NSs) were prepared by using an ultrasonic homogenization technique. The mean particle size of Cur-NSs was 210.9 nm and significantly ( < 0.05) increased to 368.8 nm by CS coating and decreased to 172.8 nm by OCS coating. Encapsulation efficiencies of Cur-NSs, CS-Cur-NSs, and OCS-Cur-NSs were 80.6%, 91.4%, and 88.5%, respectively. The mucin adsorption of Cur-NSs was steeply increased about 3-4 times by CS and OCS coating. Morphological changes of these NSs were studied using circular dichroism spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, and transmission electron microscopy (TEM). Thus, CS-Cur-NSs and OCS-Cur-NSs showed great potential as mucoadhesive nano-carriers for the efficient delivery of water insoluble compounds like curcumin to the gastrointestinal system.

采用超声均质技术制备了姜黄素纳米混悬液（Cur-NSs）、壳聚糖包衣姜黄素纳米混悬液（CS-Cur-NSs）和低聚壳聚糖包衣姜黄素纳米混悬液（OCS-Cur-NSs）。Cur-NSs的平均粒径为210.9 nm，经壳聚糖包衣后显著（<0.05）增大至368.8 nm，经低聚壳聚糖包衣后减小至172.8 nm。Cur-NSs、CS-Cur-NSs和OCS-Cur-NSs的包封率分别为80.6%、91.4%和88.5%。壳聚糖和低聚壳聚糖包衣使Cur-NSs对黏蛋白的吸附急剧增加约3 - 4倍。利用圆二色光谱、傅里叶变换红外（FT-IR）光谱和透射电子显微镜（TEM）研究了这些纳米混悬液的形态变化。因此，CS-Cur-NSs和OCS-Cur-NSs作为黏膜黏附纳米载体具有巨大潜力，可将姜黄素等水不溶性化合物有效递送至胃肠道系统。