Wei Zheng, Luo Jianming, Huang Yu, Guo Wenfeng, Zhang Yali, Guan Huan, Xu Changmou, Lu Jiang
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Int J Mol Sci. 2017 Mar 14;18(3):631. doi: 10.3390/ijms18030631.
Polyphenol compositions and concentrations in skins and seeds of five muscadine grapes (cv. "Noble", "Alachua", "Carlos", "Fry", and "Granny Val") cultivated in the United States (Tallahassee-Florida, TA-FL) and South China (Nanning-Guangxi, NN-GX and Pu'er-Yunnan, PE-YN) were investigated, using ultra performance liquid chromatography tandem triple quadrupole time-of-flight mass spectrometry (UPLC Triple TOF MS/MS). Fourteen ellagitannins were newly identified in these muscadine grapes. The grapes grown in NN-GX accumulated higher levels of ellagic acid, methyl brevifolin carboxylate, and ellagic acid glucoside in skins, and penta-O-galloyl-glucose in seeds. In PE-YN, more flavonols were detected in skins, and higher contents of flavan-3-ols, ellagic acid, and methyl gallate were identified in seeds. Abundant seed gallic acid and flavonols were found among the grapes grown in TA-FL. Based on principal component analysis (PCA) of 54 evaluation parameters, various cultivars grown in different locations could be grouped together and vice versa for the same cultivar cultivated in different regions. This is the result of the interaction between genotype and environmental conditions, which apparently influences the polyphenol synthesis and accumulation.
采用超高效液相色谱串联三重四极杆飞行时间质谱（UPLC Triple TOF MS/MS），对在美国（佛罗里达州塔拉哈西，TA-FL）和中国南方（广西南宁，NN-GX和云南普洱，PE-YN）种植的五个圆叶葡萄品种（“贵族”、“阿拉楚阿”、“卡洛斯”、“弗莱”和“瓦尔奶奶”）果皮和种子中的多酚成分及含量进行了研究。在这些圆叶葡萄中，新鉴定出了14种鞣花单宁。在NN-GX种植的葡萄果皮中积累了较高水平的鞣花酸、短叶松萝酸甲酯和鞣花酸葡萄糖苷，种子中积累了五-O-没食子酰葡萄糖。在PE-YN，果皮中检测到更多的黄酮醇，种子中鉴定出较高含量的黄烷-3-醇、鞣花酸和没食子酸甲酯。在TA-FL种植的葡萄中发现了丰富的种子没食子酸和黄酮醇。基于对54个评估参数的主成分分析（PCA），不同地点种植的不同品种可以归为一组，反之，同一品种在不同地区种植也可归为一组。这是基因型与环境条件相互作用的结果，显然影响了多酚的合成和积累。
